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Method for removing monosaccharide component from oligomerization galactose by utilizing immobilized yeast

A technology for immobilizing yeast and galacto-oligosaccharides, which is applied in the direction of immobilizing on/in organic carriers, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of difficult collection, short lifespan of free yeast cells, difficult re-use etc.

Active Publication Date: 2008-07-09
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this free yeast cell has a short lifespan and is not easy to collect and reuse, which increases the cost of industrialization. Therefore, it is necessary to find a more effective and economical method for removing the monosaccharide components in GOS

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  • Method for removing monosaccharide component from oligomerization galactose by utilizing immobilized yeast

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Embodiment Construction

[0006] A method for removing monosaccharide components in galacto-oligosaccharides by using immobilized yeast, comprising preparing immobilized yeast cells and using the immobilized yeast cells to process galacto-oligosaccharides solution, the specific steps are as follows:

[0007] 1) Preparation of immobilized yeast cells:

[0008] (1) inoculate the yeast strain in 10 degrees Baume (°Bé) wort medium and cultivate it for 18-36 hours, the culture temperature is 26-30°C, and the shaker speed is 150 rpm;

[0009] (2) Centrifuge the culture medium at 8000 rpm for 10 minutes to obtain yeast cells;

[0010] (3) The above-mentioned yeast cells collected by centrifugation were immobilized by calcium alginate embedding method to obtain immobilized yeast cells.

[0011] The prepared immobilized yeast cells were placed in 1% (w / w) glucose solution and stored at 4°C for later use;

[0012] 2) Immobilized yeast cells process the galacto-oligosaccharide solution:

[0013] Immobilized ye...

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Abstract

The invention discloses a method for removing monosaccharide components from oligo-galactose through using immobilized yeast, which comprises the following steps: taking calcium alginate as carrier to prepare yeast cells into immobilized cells, applying the cells and adopting batchwise or column-type continuous fermentation method to process oligo-galactose solution, and completely removing dextrose and lowering the content of galactose. In the method which is adopted by the invention, the immobilized yeast cells can be repeatedly used for many times and the process is simple, secure and reliable and greatly reduces the cost of production, can be applied in industrial scale, and increases the content of metastasis oligosaccharide in oligo-galactose.

Description

technical field [0001] The invention relates to a method for removing monosaccharide components in galacto-oligosaccharides, in particular to a method for removing monosaccharide components in galacto-oligosaccharides by using immobilized yeast cells. technical background [0002] Galactooligosaccharides (GOS) is an eye-catching one of many functional oligosaccharides. It has many beneficial functions, such as reducing the number of Clostridium, producing more short-chain fatty acids, and producing less gas. outstanding advantages. Commercialized GOS is to use microbial β-galactosidase to use lactose as raw material to generate glycosylation, and the product obtained is a mixture, and its components include glucose, galactose, lactose, transfer oligosaccharides (for example: Japanese Yakult Honsha company produces GOS commodity oligomate 55  The composition is 18% of glucose, 1% of galactose, 24% of lactose, and 57% of transfer oligosaccharides including transfer disaccha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N1/16C12N11/04C12R1/865
Inventor 肖敏李正义卢丽丽
Owner SHANDONG UNIV