Method for controlling release velocity of fluorescent probe molecule in emulsions

A technology of fluorescent probe and release rate, which is applied in emulsion transportation, material analysis by optical means, and material analysis, etc. It can solve the problems that it is difficult to accurately measure the release rate of materials.

Inactive Publication Date: 2008-07-30
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to accurately measure the release rat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] First add the aqueous solution of the fluorescent probe molecule (fluorescein isothiocyanate-labeled bovine serum albumin FITC-BSA or sodium fluorescein, etc.) to the Sorbitan monooleate Span80 containing 5% non-ionic surfactant In the mineral oil phase (volume ratio is 1: 19 to 1: 10, the total volume is 1 milliliter), at 273K, 9500 rev / min speed of stirring at a constant speed for 5 minutes to obtain a water-in-oil emulsion (W / O emulsion), Then add 0.8 milliliters of an aqueous solution containing 2.7% polyoxyethylene sorbitan laurate Tween20 to the prepared W / O emulsion for secondary emulsification, and emulsify at 273 K for 2 minutes at a speed of 8000 rpm to obtain a water-in-water emulsion. Water-in-oil double emulsion (W / O / W emulsion). Take 10 microliters of water-in-oil-in-water emulsion and dilute it in 1 milliliter of aqueous solution, and then analyze the sample by a fluorescence-activated cell sorter to obtain the fluorescence intensity of the fluorescent pr...

Embodiment 2

[0019] First, the water-soluble polymer (sodium carboxymethylcellulose, polyoxyethylene, etc.)-fluorescent probe (fluorescein isothiocyanate-labeled bovine serum albumin FITC-BSA or sodium fluorescein, etc.) molecular aqueous solution is used as the water phase Join in the mineral oil phase (volume ratio is 1: 19 to 1: 10, total volume is 1 milliliter) containing surfactant (5% sorbitan monooleate Span80), at 273K, 9500 rpm Stir at a constant speed for 1-5 minutes at a rotating speed to obtain a water-in-oil emulsion (W / O emulsion), and then add 0.8 milliliters of an aqueous solution containing 2.7% polyoxyethylene sorbitan laurate Tween20 to the prepared W / O emulsion. Secondary emulsification, emulsification at 273K8000 rpm for 2 minutes to obtain a water-in-oil-in-water double emulsion (W / O / W emulsion). Take 10 microliters of water-in-oil-in-water emulsion and dilute it in 1 milliliter of aqueous solution, and then analyze the sample by a fluorescence-activated cell sorter t...

Embodiment 3

[0021] Using oil-in-water emulsion (O / W), dissolve anticancer drugs (hydrophobic materials such as paclitaxel PTX) in the oil phase (vegetable oil or salad oil), and add to the mixture containing 3%-6% nonionic surfactant ( Preferred food grade Tween series polyoxyethylene sorbitan laurate Tween20) in the aqueous solution, at 273K under the rotating speed of 9500 rev / mins, prepare into O / W emulsion. By changing the volume ratio of the water phase to the oil phase, O / W stable emulsions containing different concentrations of anticancer drugs in the oil phase were prepared respectively, and the fluorescence intensity and concentration of fluorescent probe molecules and anticancer drugs were analyzed by fluorescence-activated cell sorting. The size and stability of O / W droplets achieve the dual purpose of controlling the release of anticancer drugs and obtaining the release mechanism of anticancer drugs.

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Abstract

A method for controlling the speed of releasing the fluorescent probe molecules in the latex emulsion belongs to the material controlled release technical field. The art is as follows: a fluorescent probe molecule water solution or a polymer-fluorescent probe molecule water solution taken as the water phase is added into a mineral oil phase containing a polylol non-ionic surface promoting agent, the volume ratio of the polylol non-ionic surface promoting agent to the mineral oil ranges from 1:19 to 1:10, the solution is evenly stirred at a rotating speed of 8000 to 12000 turns per minute under 273 to 298 K for 1 to 5 minutes to get a water-in-oil latex emulsion, a 0.8 milliliters of water solution containing two to four percent of polyoxyethylene sorbitan laurate is added into to the prepared water-in-oil latex emulsion to be emulsionized for the second time for 2 to 5 minutes to obtain the water-in-oil or oil-in-water latex double emulsion. The sample is analyzed by a fluorescent energetic cell sorter and the release speed of the fluorescent probe molecules is calculated by a formula. The invention has the advantages of high speed, high efficiency, directness and accuracy.

Description

technical field [0001] The invention belongs to the technical field of material release control, in particular to a method for controlling the release rate of fluorescent probe molecules in emulsion, which is suitable for polymer materials. Background technique [0002] Controlled release of materials is very important in the fields of controlled and sustained release of drugs, proteins and cosmetics. However, it is difficult to accurately measure the release rate of materials from emulsions by analytical detection methods. Fluorescence-activated cell sorting (FACS: a commonly used instrument in biochemical research) is a fast, direct, and accurate determination of fluorescent probe molecules (including solutes such as drugs, proteins, genes, etc.) from water-in-oil-in-water A new method of release rate in emulsion (W / O / W emulsion) or oil-in-water emulsion (O / W), polymer can control the release rate of fluorescent probe molecules from W / O / W emulsion, so as to achieve materi...

Claims

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Application Information

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IPC IPC(8): G01N21/00G01N33/48A61K9/107A61K31/337A61P35/00
Inventor 海明潭孔峰
Owner UNIV OF SCI & TECH BEIJING
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