Device for observing magnetism tendency bacteria habit

Abstract

The invention relates to a viewing device of magnetotactic bacteria habit, which belongs to the microbiology field. The invention is provided with a magnetotactic bacteria bacterial suspension container, which is characterized in that the container is a transparent cuboid structure, the left portion and the right portion of the container are respectively provided with a left magnet coil and a right magnet coil, one end of the left magnet coil is connected with the positive pole of a direct current power supply, and the other end of the left magnet coil is connected with the negative pole of the direct current power supply through a slide rheostat R1, one end of the right magnet coil is connected with the positive pole of the direct current power supply through a switch K1, and the other end of the right magnet coil is connected with the negative pole of the direct current power supply through a switch K2 and a slide rheostat R2. The strength and direction of electromagnetic field in the container is changed through adjusting resistance value of the slide rheostat and the current direction of the left and the right magnet coil, which are convenient for viewing growth habit of magnetotactic bacteria in different magnetic field conditions under oxygen dissolution condition.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to a cultivation device for magnetotactic bacteria. Background technique [0002] Magnetotactic bacteria are a class of Gram-negative bacteria with flagella that can move. They are widely distributed and can be found in lakes, oceans and even wet soils. Its most important morphological feature is the intracellular magnetosome chain, which refers to the magnetic particles surrounded by membranes generated in the cells of magnetotactic bacteria. Each magnetotactic bacterial cell contains one or more magnetosome chains, which are located close to the cell wall and generally distributed along the long axis of the cell. The magnetosomes of most magnetotactic bacteria have relatively high purity and are Fe-containing 3 o 4 Ferrite magnetic particles, a few of which contain Fe 3 S 4 Iron-sulfur magnetic particles, the size of a single particle is between 35-120nm. It has a large specific ...

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Problems solved by technology

[0003] Although magnetotactic bacteria exist in a wide range and in large numbers, it is very difficult to cultivate magnetotactic bacteria and obtain magnetosomes in the laboratory, because many magnetotactic bacteria are sensitive to extremely low concentrations of oxygen, and most of them are artificially cultured. Magnetosomes can only be produced in a very narrow range with very low concentrations of oxygen, which has been an important limiting factor for this field of research for many years

[0004] Magnetotactic bacteria can move along the direction of the magnetic field and the direction of the oxygen concentration gradient. The synergistic effect of magnetotacticity and oxygenotropy is actually a "magnetotactic-oxygenotropic" behavior. Analyze and grasp the relationship between the strength and direction of the magnetic field in the medium and The optimal combination of dissolved oxygen content is a crucial problem in the cultivation of magnetotactic bacteria in the laboratory. At present, a single fixed magnet is used in this research. The strength and direction of the magnetic field cannot be adjusted, which has great limitations. It is difficult to achieve the desired effect in

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