Edible fungi sclerotium oyster mushroom strain and application thereof
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A technology of strains and sclerotia, which is applied in the field of Sclerotinia sclerotiorum Donghua Tiger Milk-No. 1 strain, can solve the problem of no strains and the like
Inactive Publication Date: 2008-08-13
EAST CHINA UNIV OF TECH
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Problems solved by technology
The distribution of the wild fungus Pleurotus sclerotiorum was only recorded in Yunnan Province of my country in the past, and the large-scale artificial domestication and cultivation of wild strains and related technical parameters are still in the exploratory stage in China
For a long
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Embodiment 1
[0023] 1. Strain preparation
[0024] The mother species is cultured with PDA enriched medium, medium: PDA+yeast extract (5g / L)+(KH 2 PO 4 1g / L, VB 1 0.01g / L, MgSO 4 0.5g / L), pH6.5 formulation, prepared by conventional methods.
[0025] Original seed cultivation: The original seed is barley seed, and the formula of barley seed is: 5000g of wheat, 150g of gypsum powder, and appropriate amount of water; the production process is: take the barley and boil it until there is no white heart, cool until it is not sticky, and mix it with gypsum Powder, bottled and sterilized, then inoculated into the parent species, and cultured at 25°C for about 25 days.
[0026] 2. Production of bacteria tube
[0027] Cultivation material formula: 84% cottonseed hulls, 14% bran, 1% lime, 1% gypsum, and 60% water content; after mixing the above dry materials in proportion, put them into a 55-60cm×17cm×0.05cmm Bacteria bags, after normal pressure sterilization and cooling, punch holes (4 holes p...
Embodiment 2
[0036] 1. Strain preparation
[0037] The mother species adopts PDA+yeast extract (5g / L)+(KH 2 PO 4 1g / L, VB 1 0.01g / L, MgSO 4 0.5g / L), pH6.5 formula, prepared by conventional methods;
[0038] The original seed is a liquid strain, and the medium formula is: wheat bran 100g + glucose 15g + sucrose 5g + peptone 4g, add water to 1000mL. After bottling and sterilizing, inoculate the parent species, and cultivate at 25°C for about 25 days.
[0039] For subsequent steps: the production of the bacteria tube, the management of the growth of bacteria and the management of the fruiting of mushrooms covered with soil in the awning are the same as the method in Example 1.
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Abstract
The invention discloses a sclerotium oyster-cap fungus donghua tiger milk number 1bacterial strain and it application. The bacterial strain belongs to basidiomycetes oyster-cap fungus section, alternate name is tiger-milk bacterium, nuclear ear mushroom,poria cocos wolf oyster-cap fungus, nanyang oyster-cap fungus and it is preserved on September 25th, 2005 year whose entreasure identification serial number is CGMCC No.1545. It has strong anti- polygamy, bacterial filament upgrowth temperature is 18 to 38 DEG C and the most suitable temperature is 24 to 27 DEG C, bacterial filament upgrowth ph is 5.5 to 6.8 and the most suitable Ph is 5.8 to 6.5. The bacterial strain is a typical high temperature variety which can grow in simple shade in hot summer. The fresh mushroom has delicious flavor, good mouthfeel and keeping quality identity and the dried mushroom has good color and fragrance. The sclerotium oyster-cap fungus donghua tiger milk number 1 has abundant nutrition ingredient and is not only edible but also a treasure dual purpose large scale fungi both for food and medicine. Also the fungi is an excellent health care food and has the function of prolong life and health care beauty treatment for elder on condition of long-term consumption.
Description
technical field [0001] The invention relates to an edible fungus, in particular to a Pleurotus sclerotium Donghuahumilk-1 strain and application thereof. Background technique [0002] Edible fungi refer to large fungi that can be eaten by humans. At present, there are more than 720 kinds of edible fungi reported in my country, but only more than 50 species have been domesticated and cultivated. Among these varieties, except straw mushrooms and a few high-temperature oyster mushroom varieties that can produce mushrooms under relatively high-temperature natural conditions, the others basically only produce mushrooms in winter and spring; although straw mushrooms and a few oyster mushroom varieties can also produce mushrooms under relatively high-temperature conditions Fruiting, but their yield and shelf life are limited, and they are not resistant to storage. Therefore in summer and early autumn, especially in scorching summer, there are seldom fresh mushrooms coming into the...
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