Edible fungi sclerotium oyster mushroom strain and application thereof
A technology of strains and sclerotia, which is applied in the field of Sclerotinia sclerotiorum Donghua Tiger Milk-No. 1 strain, can solve the problem of no strains and the like
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Embodiment 1
[0023] 1. Strain preparation
[0024] The mother species is cultured with PDA enriched medium, medium: PDA+yeast extract (5g / L)+(KH 2 PO 4 1g / L, VB 1 0.01g / L, MgSO 4 0.5g / L), pH6.5 formulation, prepared by conventional methods.
[0025] Original seed cultivation: The original seed is barley seed, and the formula of barley seed is: 5000g of wheat, 150g of gypsum powder, and appropriate amount of water; the production process is: take the barley and boil it until there is no white heart, cool until it is not sticky, and mix it with gypsum Powder, bottled and sterilized, then inoculated into the parent species, and cultured at 25°C for about 25 days.
[0026] 2. Production of bacteria tube
[0027] Cultivation material formula: 84% cottonseed hulls, 14% bran, 1% lime, 1% gypsum, and 60% water content; after mixing the above dry materials in proportion, put them into a 55-60cm×17cm×0.05cmm Bacteria bags, after normal pressure sterilization and cooling, punch holes (4 holes p...
Embodiment 2
[0036] 1. Strain preparation
[0037] The mother species adopts PDA+yeast extract (5g / L)+(KH 2 PO 4 1g / L, VB 1 0.01g / L, MgSO 4 0.5g / L), pH6.5 formula, prepared by conventional methods;
[0038] The original seed is a liquid strain, and the medium formula is: wheat bran 100g + glucose 15g + sucrose 5g + peptone 4g, add water to 1000mL. After bottling and sterilizing, inoculate the parent species, and cultivate at 25°C for about 25 days.
[0039] For subsequent steps: the production of the bacteria tube, the management of the growth of bacteria and the management of the fruiting of mushrooms covered with soil in the awning are the same as the method in Example 1.
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