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Method for preparing vanadium-containing peptides and products produced thereby

The technology of polypeptide powder and polypeptide liquid is applied in the field of preparation of biological raw materials, which can solve the problems such as no records of vanadium-containing polypeptides and vanadium-containing small peptide products, save energy and water, facilitate industrial production, and improve protein hydrolysis. rate effect

Inactive Publication Date: 2011-09-14
秋生堂生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In contemporary times, although peptide chelates (SPC) of various metal elements prepared according to this mechanism have been widely used in animal feeding as the fourth generation of trace element preparations, there are no vanadium-containing peptides and vanadium-containing small peptide products at home and abroad. record

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Prepare vanadium-rich soybeans according to the method of No. 200710302099.0 Chinese patent application

[0047] (1) Use 450mg sodium orthovanadate, 10g gibberellin, 10g potassium bromate and 100 liters of water to prepare the initial soaking solution. Wash the soybeans, put them into the initial soaking liquid at a ratio of 1:3 (W / V), adjust the constant temperature between 16-37° C., soak for 8-15 hours, separate the soybeans from the soaking liquid, and obtain the initial soaking soybeans. The soaking liquid collected by separation can be kept for another use.

[0048] (2) Evenly spray 0.1% potassium bromate aqueous solution on the soaked soybeans for the first time, and place them at a temperature of 16-37°C, a relative humidity of 60-95%, and a temperature of 300-1000M 3 / (t·h) air volume and constant oxygen incubator for 8-10 hours to obtain soybeans for the first time.

[0049] (3) Add 150 g of sodium orthovanadate, 5 g of gibberellin, and 5 g of pota...

Embodiment 2

[0053] Embodiment 2 mutagenesis method cultivates high-activity vanadium-rich fermentation strain

[0054] 1. Strain selection

[0055] Use any edible microorganism suitable for protein hydrolysis, such as Bacillus, Rhizopus chinensis, Actinomyces elegans, Monascus, Aspergillus niger, Actinomyces elegans, Bacillus subtilis, Bacillus natto, Actinomyces, Bacillus, Aspergillus Usami mutant strains, Aspergillus cinnamon, Aspergillus oryzae, Saccharomyces cerevisiae, Lactobacillus bulgaricus, etc. Among them, Bacillus sp. CAU208 is preferred.

[0056] 2. Preparation of culture medium

[0057] Add vanadium compound to prepare four kinds of culture medium, namely, activation medium, seed medium, separation medium and fermentation medium, and use them in different production links.

[0058] ①Activation medium: used to activate the vanadium tolerance of cultured microorganisms. The materials and proportions are: peptone 0.25-0.75%, glucose 0.25-0.75%, yeast extract 0.25-0.75%, potas...

Embodiment 3

[0071] Example 3 Preparation of Soybean Flour Rich in Organic Vanadium and Soybean Peptides by Solid State Fermentation

[0072] Using ordinary soybeans as raw materials, or using vanadium-rich soybeans prepared according to the method of Example 1 as raw materials, first wash with an acidic aqueous solution with a pH of 3.0-7.5 prepared with 1-7% citric acid or other edible acids, and then rinse with water , drained, spray 50-500ml of 10-50% vanadium compound aqueous solution per kilogram, and stir evenly. Steam at 100-125°C for 30-60 minutes, cool to room temperature, spray soybean peptide, and inoculate the high-efficiency vanadium-rich fermentation strain selected and cultivated according to the method in Example 2 into the cooked and cooled material according to the inoculation amount of 1-10%. Cultivate at a temperature of 20-40°C, according to the air volume of 1000-3000M 3 / (t·h) Ventilate, turn the material once every 16 hours, and stop fermentation after the beans a...

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PUM

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Abstract

The invention discloses a process for preparing peptide which comprises vanadium, which comprises using stewing material which is rich in protein after scouring with acidulous water solution, mixing with vanadate, using soybean peptide as fermentation accelerating agent, grafting fermenting bacterial strain into raw material which is stewed, fermenting for 6-72h under 20-40 DEG C, washing, drying, disintegrating in ultrafine to obtain vanadium-enriched polypeptide powder, removing peptide with large molecular to obtain vanadium-enriched liquid peptide, and chelating through adding vanadate toobtain vanadium-enriched peptide whose peptide concentration is over 60%, and vanadium element concentration is over 100 mg / kg.

Description

technical field [0001] The present invention relates to the preparation method of biological raw material for food and medicine processing, especially the preparation method of peptide containing organic vanadium; the present invention also relates to the product prepared according to the method. Background technique [0002] Vanadium is one of the essential trace elements for human beings. Whether it is inorganic vanadium, organic vanadium, or biotransformation vanadium, as long as it can be absorbed under the safe limit, it can produce medical and health effects. [0003] The absorption rate of inorganic vanadium by organisms is very low, the maximum absorption rate of cations is 10%, and the absorption rate of anions is only 0.01-0.1%. At the same time, inorganic vanadium has high toxicity and side effects, and the effective dose for human treatment is 100-1000 times that of animal experiments. Therefore, the currently known inorganic vanadium cannot be used clinically. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A23J3/14A23J3/16A23L1/305C12R1/07A23L33/16A23L33/18
Inventor 卢广荣
Owner 秋生堂生物科技(北京)有限公司
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