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Hydrolyzed protein preparation method

A technology for hydrolyzing protein and collagen, applied in the field of collagen extraction, can solve the problems that inorganic zinc cannot be used clinically, inorganic zinc has large toxic and side effects, etc., and achieves the effects of preventing or alleviating diabetes complications, eliminating bitterness, and low enzymatic hydrolysis temperature.

Active Publication Date: 2015-07-08
HUBEI RUIBANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

At the same time, inorganic zinc has high toxicity and side effects, and the effective dose for human body treatment is 100-1000 times that of animal experiments.
[0007] Therefore, currently known inorganic zinc cannot be used clinically.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for preparing hydrolyzed protein, comprising the steps of:

[0031] Cleaning steps: Put the fish skin into the cleaning tank, then add clean water into the cleaning tank, and then ultrasonically stir the cleaning tank for 30-60 minutes; wherein, the amount of clean water added is 30% to 120% of the weight of the fish skin;

[0032] Enzymolysis step: Put the fish skin in a meat grinder and grind it to get a slurry with a particle size of less than 0.6 cubic centimeters, pour the slurry into the enzymatic hydrolysis reaction tank, and add deionized water 0.1 to 0.5 times the weight of the fish skin , and add 0.1~0.5% of the weight of fish skin acid protease, then heat up the enzymolysis reaction tank to 30~50°C while stirring, and enzymolysis for 2~5 hours, while controlling the pH value in the enzymolysis reaction tank to 2.5 ~6;

[0033] Heavy metal removal step: remove the heavy metal from the enzymatically hydrolyzed fish skin; put the enzymatically hydrolyz...

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PUM

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Abstract

The invention belongs to the field of collagen preparation, and discloses a hydrolyzed protein preparation method, a hydrolyzed protein peptide product and a preparation method of the hydrolyzed protein peptide product. The hydrolyzed protein preparation method comprises the following technological steps: cleaning fish skin with water, mincing the fish skin so as to obtain slurry, carrying out enzymolysis on the fish skin slurry, carrying out enzyme deactivation treatment at 100 DEG C, filtering so as to obtain a collagen solution, carrying out activated carbon decoloration treatment, concentrating so as to obtain collegen. The preparation technology is simple, has no environmental pollution, and is low in production cost.

Description

technical field [0001] The invention relates to the technical field of collagen extraction, in particular to a method for preparing hydrolyzed protein. Background technique [0002] Collagen is a kind of protein, which mainly exists in the skin, bone, tendon, ligament and blood vessel of human body and land animals. However, due to the prevalence of some highly contagious mad cow disease, bird flu, foot-and-mouth disease and other infectious diseases in terrestrial animals recently, people have questioned the safety of collagen extracted from these terrestrial animals. [0003] In recent years, collagen researchers have begun to pay attention to extracting collagen from aquatic animals, such as extracting collagen from fish scales. However, the collagen extraction process in the existing fish scales mainly uses industrial hydrochloric acid to clean, and then cleans with alkaline solution, such as sodium hydroxide solution. The introduction of a large amount of acid and alk...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 尹晓清刘淑霞魏辉平邓洁李丽萍张雨李荣维黄飞刘差娥
Owner HUBEI RUIBANG BIOTECHNOLOGY CO LTD
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