Magnetic tumor double-target polymer nano micelle and preparation thereof
A nanomicelle, dual-targeting technology, applied in the fields of polymer chemistry and biomedical engineering, can solve the problems of difficult particle size control, low drug loading rate, poor release effect, etc. Excellent effect on blood circulation time and chain compliance
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Embodiment 1
[0029] Preparation of folate-PEG-PCL or folate-PEG-PLA carrier material folate-PEG-PCL or folate-PEG-PLA for tumor dual-targeting nanomicelles modified by folic acid
[0030] (1) Preparation of allyl-terminated PEG homopolymer
[0031]Mix 4ml of tetrahydrofuran solution of potassium naphthyl with 0.5ml of allyl alcohol and stir in a dry reaction bottle for 15 minutes. Then add 20ml of anhydrous tetrahydrofuran and 18-crown ether-6 tetrahydrofuran solution (containing 1.5g 18-crown ether-6 and 5ml anhydrous tetrahydrofuran) under the protection of argon, and after stirring for 15 minutes, place the mixture in an ice-salt bath Cool, and slowly pass through dry ethylene oxide, and maintain low temperature for 24 hours so that the polymerization reaction continues. Finally, it was left at room temperature for 3 days.
[0032] (2) Preparation of allyl-PEG-PCL or allyl-PEG-PLA amphiphilic block copolymer terminated by allyl group
[0033] Under the protection of argon, dry 0.2g o...
Embodiment 2
[0039] Preparation of tumor dual-targeting polymer nano drug-loaded micelles
[0040] (1) Preparation of superparamagnetic Fe3O4 nanoparticles SPIO
[0041] Mix 0.7g iron acetylacetonate, 2.9g 1,2-hexadecanediol, 2ml oleic acid, 2ml oleylamine and 20ml dibenzyl ether under nitrogen protection and heat at 200°C for two hours, then raise the temperature to 300°C Reflux for one hour. After the obtained black product was cooled to room temperature, it was reprecipitated in ethanol, centrifuged to remove excess solvent, dissolved in n-hexane and sealed for storage.
[0042] (2) Preparation of tumor dual-targeting polymer nano drug-loaded micelles
[0043] Dissolve 10mg of folate-PEG-PCL or folate-PEG-PLA, 2mg of anticancer drugs, 1.3ml of triethylamine, and 1.5mg of SPIO in a mixed solvent containing 1.0ml of tetrahydrofuran and 1.0ml of dimethyl sulfoxide. Slowly added dropwise to 5ml deionized water. Then, they were dialyzed in deionized water for 2 days to remove organic sol...
Embodiment 3
[0045] Test experiment of basic properties of tumor dual-targeting polymer nano drug-loaded micelles
[0046] The particle size of the obtained micelles is measured by a dynamic light scattering system, and its shape is determined by observation with a transmission electron microscope. The test results are shown in figure 1 .
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