Substrate for biochip, biochip, method for manufacturing substrate for biochip, and method for manufacturing biochip

A biochip and manufacturing method technology, applied in biochemical equipment and methods, biological material pretreatment, biological testing, etc., can solve problems such as the difficulty of immobilizing probe DNA and the reduction of DNA chip detection accuracy

Inactive Publication Date: 2008-09-10
YAMATAKE HONEYWELL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, immobilizing probe DNA only at specific positions is difficult
Therefore, in the detection process, the target DNA that reacts with the probe DNA bound to other than the specific position becomes background noise and becomes a factor that lowers the detection accuracy of the DNA chip.

Method used

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  • Substrate for biochip, biochip, method for manufacturing substrate for biochip, and method for manufacturing biochip
  • Substrate for biochip, biochip, method for manufacturing substrate for biochip, and method for manufacturing biochip
  • Substrate for biochip, biochip, method for manufacturing substrate for biochip, and method for manufacturing biochip

Examples

Experimental program
Comparison scheme
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no. 1 Embodiment approach

[0071] The biochip substrate according to the first embodiment of the present invention, as shown in FIG. 1 and FIG. 2 which is a cross-sectional view viewed from the II-II direction, has a substrate 15 capable of introducing a plurality of hydroxyl groups (-OH) on the surface. , and configured on the substrate 15, provided with a plurality of holes 41a, 41b, 41c, 41d, 41e, 41f, 41g, 41h, 41i, 42a, 42b, 42c, 42d, 42e, 42f, 42h reaching the substrate 15, 42i, 43a, 43b, 43c, 43d, 43e, 43f, 43g, 43h, 43i, 44a, 44b, 44c, 44d, 44e, 44f, 44g, 44h, 44i, 45a, 45b, 45c, 45d, 45e, 45f, 45g, 45h, 45i, 46a, 46b, 46c, 46d, 46e, 46f, 46g, 46h, 46i, 47a, 47b, 47c, 47d, 47e, 47f, 47g, 47h, 47i, 48a, 48b, 48c, 48d, Metal-based films 13 of 48e, 48f, 48g, 48h, 48i, 49a, 49b, 49c, 49d, 49e, 49f, 49g, 49h, 49i. The film thickness of the substrate 15 is, for example, 550 μm, and as the material of the substrate 15, synthetic quartz (SiO 2 ) and a silicon substrate with a natural oxide film formed...

no. 2 Embodiment approach

[0080] The substrate for a biochip according to the second embodiment of the present invention, as shown in FIG. 7 and FIG. 8 , which is a sectional view viewed from the direction III-III, includes a base 15, and a base 15 arranged on the base 15 is provided with a base that reaches the base. 15 a plurality of holes 41a, 41b, 41c, 41d, 41e, 41f, 41g, 41h, 41i, 42a, 42b, 42c, 42d, 42e, 42f, 42h, 42i, 43a, 43b, 43c, 43d, 43e, 43f , 43g, 43h, 43i, 44a, 44b, 44c, 44d, 44e, 44f, 44g, 44h, 44i, 45a, 45b, 45c, 45d, 45e, 45f, 45g, 45h, 45i, 46a, 46b, 46c, 46d , 46e, 46f, 46g, 46h, 46i, 47a, 47b, 47c, 47d, 47e, 47f, 47g, 47h, 47i, 48a, 48b, 48c, 48d, 48e, 48f, 48g, 48h, 48i, 49a, 49b , 49c, 49d, 49e, 49f, 49g, 49h, 49i metal film 13. The respective film thicknesses and materials of the substrate 15 and the metal-based film 13 are the same as those of the biochip substrate shown in FIG. 1 , and thus description thereof will be omitted.

[0081]Furthermore, the biochip according to the...

no. 3 Embodiment approach

[0107] The biochip substrate shown in the third embodiment, as shown in FIG. 30 and FIG. 31 , which is a cross-sectional view viewed from the X X XI-XX XI direction, has a substrate 15 capable of introducing a plurality of hydroxyl groups on the surface, and a probe A plurality of through-holes 141a, 141b, 141c, 141d, 141e, 141f, 141g, 141h, 141i are disposed on the substrate 15 when biomolecules are bound to a plurality of hydroxyl groups, and define the binding regions of the probe biomolecules on the surface of the substrate 15. , 142a, 142b, 142c, 142d, 142e, 142f, 142h, 142i, 143a, 143b, 143c, 143d, 143e, 143f, 143g, 143h, 143i, 144a, 144b, 144c, 144d, 144e, 144f, 144g, 144h , 144i, 145a, 145b, 145c, 145d, 145e, 145f, 145g, 145h, 145i, 146a, 146b, 146c, 146d, 146e, 146f, 146g, 146h, 146i, 147a, 147b, 147c, 147d, 147e, 147f , 147g, 147h, 147i, 148a, 148b, 148c, 148d, 148e, 148f, 148g, 148h, 148i, 149a, 149b, 149c, 149d, 149e, 149f, 149g, 149h, 149i of the protective part 2...

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Abstract

Disclosed is a substrate for biochips which comprises a base to the surface of which a plurality of hydroxyl groups can be introduced, a metal film arranged on the base and provided with a plurality of wells reaching the base, and a crosslinkable polymer film arranged on the metal film.

Description

technical field [0001] The present invention relates to detection technology of biomolecules, and particularly relates to a substrate for a biochip, a biochip, a method for manufacturing a substrate for a biochip, and a method for manufacturing a biochip. Background technique [0002] The so-called biochip is a general term for elements in which probe biomolecules that specifically bind to target biomolecules to be detected are immobilized at specific positions on the surface of a substrate. A deoxyribonucleic acid (DNA) chip, which is a representative example of a biochip, is used to detect the type and amount of target DNA contained in blood or cell extracts. In the case of a DNA chip, for example, on a substrate such as a glass slide, several thousand to tens of thousands of probe DNAs, which are single-stranded DNAs with known sequences, are arranged in an array. When the test solution containing fluorescently labeled target DNA is supplied to the DNA chip, only the tar...

Claims

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Application Information

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IPC IPC(8): G01N33/53C12M1/00C12N15/09G01N37/00
Inventor 五所尾康博黑岩孝朗石川尚弘小原太辅山崎信介佐佐木和子堀口康子
Owner YAMATAKE HONEYWELL CO LTD
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