Plants with increased hyaluronan production

A technology of hyaluronan and plants, applied in the direction of using vectors to introduce foreign genetic material, transferase, recombinant DNA technology, etc., can solve the problems of separation or purification, such as complicated and expensive separation and purification

Inactive Publication Date: 2008-09-24
BASF AG
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  • Abstract
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Problems solved by technology

Furthermore, isolating or purifying hyaluronan from plants with low hyaluronan content is considerably more complicated and expensive than isolating and purifying from plants with high hyaluronan content

Method used

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  • Plants with increased hyaluronan production
  • Plants with increased hyaluronan production

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Experimental program
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Embodiment

[0274] 1. Preparation of plant expression vector IR 47-71

[0275]The plasmid pBinAR is a derivative of the binary vector plasmid pBin19 (Bevan, 1984, Nucl Acids Res 12: 8711-8721). It was constructed as follows: A fragment of 529 bp in length containing nucleotides 6909-7437 of the 35S promoter of cauliflower mosaic virus was isolated from the plasmid pDH51 as the EcoR I / Kpn I fragment (Pietrzak et al., 1986 Nucleic Acids Res. 14, 5858), and connected to the EcoR I and Kpn I restriction sites in the polylinker of pUC18. In this way, plasmid pUC18-35S was formed. Using restriction endonucleases Hind III and Pvu II, the T-DNA with a length of 192 bp including the Ti plasmid pTiACH5 (Gielen et al., 1984, EMBO Journal 3, 835-846) (nucleotides 11749-11939) The fragment of the polyadenylation signal (3' end) of the octopine synthase gene (gene 3) was isolated from the plasmid pAGV40 (Herrera-Estrella et al., 1983 Nature, 303, 209-213). Next, the SphI linker was added to the restriction...

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Abstract

The present invention relates to plant cells and plants which synthesize an increased amount of hyaluronan, and to methods for preparing such plants, and also to methods for preparing hyaluronan with the aid of these plant cells or plants. Here, plant cells or genetically modified plants according to the invention have hyaluronan synthase activity and additionally an increased glutamine:fructose 6-phosphate amidotransferase (GFAT) activity compared to wild-type plant cells or wild-type plants. The present invention furthermore relates to the use of plants having increased hyaluronan synthesis for preparing hyaluronan and food or feedstuff containing hyaluronan.

Description

Technical field [0001] The present invention relates to plant cells and plants with increased synthesis of hyaluronan, and a method for preparing such plants, as well as a method for preparing hyaluronan by using these plant cells or plants. Here, compared with wild-type plant cells or wild-type plants, the plant cells or genetically modified plants according to the present invention have hyaluronan synthase activity and additional glutamine: fructose 6-phosphate-amid transferase (GFAT )active. The invention also relates to the use of plants with enhanced hyaluronan synthesis in the preparation of hyaluronan and hyaluronan-containing food or feed. Background technique [0002] Hyaluronan is a naturally occurring unbranched, linear mucopolysaccharide (glucosaminoglycan), which is composed of alternate molecules of glucuronic acid and N-acetylglucosamine. The basic constituent area of ​​hyaluronan is composed of glucuronic acid disaccharide-β-1,3-N-acetylglucosamine. In hyaluronan,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N9/10
Inventor C·弗罗贝格B·埃西格曼
Owner BASF AG
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