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Automatic cell culture microflow control chip device and method thereof

A technology of microfluidic chip and cell culture, applied in the field of cell chip and its cell culture, can solve the problems of slowness, time-consuming and labor-intensive, etc., and achieve the effect of simple, convenient and efficient automatic cell culture process

Inactive Publication Date: 2008-10-15
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the traditional cell culture process is a time-consuming and labor-intensive task. If it involves dozens or even hundreds of cell cultures at the same time, it is a huge task, which almost determines the progress of research through cell culture. will be very slow

Method used

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  • Automatic cell culture microflow control chip device and method thereof
  • Automatic cell culture microflow control chip device and method thereof
  • Automatic cell culture microflow control chip device and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Microfluidic cell culture chip for automated culture of anchorage-dependent cells

[0046] The material of the chip is plastic, and it contains a 24×24 array of microculture units, and each microculture unit contains 8 microsieves, that is, the microstructure, for capturing cells. Chip structure and microsieve structure can refer to figure 1 shown. The microfluidic cell culture chip can be used to simultaneously perform automatic culture of 24 kinds of anchorage-dependent cells (24 columns), and can culture 24 microculture units for each kind of cells.

Embodiment 2

[0047] Example 2. Microfluidic cell culture chip for automated culture of suspension cultured cells

[0048] The material of the chip is silica gel, which contains a 96×96 array of microculture units, and each microculture unit contains 3 semicircular microsieves for capturing cells. Partial structure of chip and microsieve structure such as Figure 4 As shown, the microfluidic cell culture chip can be used for automatic culture of 96 types of suspension culture cells at the same time, and each type of cell can be cultured with 96 micro culture units.

[0049]In summary, the device and method of the present invention facilitate the automatic cultivation of a large number of various cells, improve the efficiency of cell culture, and reduce the time and space for culture, and are a very excellent tool for cell biochemistry research and method.

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Abstract

The invention discloses an automatic cell culture micro-fluidic chip device and a method for making the same. The device comprises a plurality of micro-culture units which are communicated in sequence and are used for receiving culture solution and cells to be cultured; and a plurality of micro structures which are arranged in each micro-culture unit and are used for capturing cells in the culture solution. The device and the method for making the same realize a high-efficient automatic cell culture process because of the adoption of the micro-culture units which are arranged in arrays, with simple and convenient realizing mode.

Description

technical field [0001] The invention relates to a cell chip and a cell culture method thereof, in particular to an automatic cell culture microfluidic chip and a cell culture method thereof. Background technique [0002] In the prior art, the cell chip is a kind of biochip technology that uses living cells as the research object, and it is produced to meet the requirements of human beings for life science exploration in the post-genome era. Cell chips not only maintain the advantages of traditional cell research methods, such as in situ detection, but also meet the requirements of high throughput, large samples, and rapid acquisition of living cell information. It enables humans to effectively use hundreds of cell lines or cell lines in nature or in a specific state to study the relationship between specific genes and their expressed proteins and diseases. It is useful for scientific research, development, disease diagnosis, Screening of drug therapeutic targets, cell local...

Claims

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Application Information

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IPC IPC(8): C12M3/02C12M1/36C12N5/00
Inventor 王站会
Owner SHENZHEN INST OF ADVANCED TECH