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Method for establishing radiation induced vitiligo mice model and application thereof

A radiation-induced, mouse model technology, applied in applications, pharmaceutical formulations, veterinary instruments, etc., to achieve the effects of fast growth, easy promotion and use, and low radiation dose

Inactive Publication Date: 2008-10-22
INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the establishment of an animal model of vitiligo induced by radiation exposure in IRM-2 mice.

Method used

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  • Method for establishing radiation induced vitiligo mice model and application thereof
  • Method for establishing radiation induced vitiligo mice model and application thereof
  • Method for establishing radiation induced vitiligo mice model and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Purpose of the experiment: Based on previous experimental observations, to investigate the establishment of vitiligo mouse model induced by radiation exposure in IRM-2 mice.

[0027] Experimental Materials:

[0028] 1. Experimental animals C57BL / 6 mice were purchased from Weitong Lihua, approval number: SCXK (Beijing) 2007-0001; IRM-2 mice were from the Institute of Radiological Medicine, Chinese Academy of Medical Sciences, approval number: SCXK (Jin ): 2005-00019. IRM-2 mice are inbred mice bred by crossing ICR / JCL mice and 615 mice according to the modified single line, and are sold to the outside world. The ICR / JCL mice were purchased from the Institute of Radiation Medicine, Chinese Academy of Medical Sciences, and the 615 mice were purchased from the Institute of Hematology, Chinese Academy of Medical Sciences.

[0029] 2. Reagent K 3 EDTA was purchased from Sigma; CD4, CD8, CD25, B220, NK1.1 were purchased from eBioscience. CD11c and Lysing Buffer were purcha...

Embodiment 2

[0069] Discussion on the immunological mechanism of radiation-induced vitiligo mice, the experimental design is the same as above.

[0070] 1. Immune cell typing in peripheral blood of healthy mice

[0071] The peripheral blood of the mice was collected for flow cytometric analysis of immune cell typing, and the results are shown in Table 4. By detecting CD4, CD8, CD25, D11b, and NK1.1, it was found that compared with C57 mice, IRM-2 mice had certain differences in CD4 and CD4 / CD8, which showed that IRM-2 mice had low CD4 / CD8 in C57 mice. CD11b is higher in IRM-2 mice than in C57 mice. There was no obvious difference between CD25 and NK1.1 mice. The significance of the difference between the two types of mouse immune cell types needs to be further explored.

[0072] Table 5 Cellular and humoral immunological indicators of healthy mice

[0073]

[0074] 2. Immune cell typing in peripheral blood of radiation-exposed mice

[0075] Blood was collected 10 weeks after radia...

Embodiment 3

[0101] Mechanism of peroxidative damage in radiation-induced skin depigmentation lesions

[0102] One week after IRM-2 mice and C57 mice were irradiated, peripheral blood was taken, serum was prepared, and SOD activity was measured. It was found that the IRM-2 of the non-irradiated group was lower than that of C57 mice; after irradiation, the SOD activity decreased. Mice decreased by about 10%, while C57 mice decreased by 30% and 40%, respectively. It suggested that the SOD activity of IRM-2 mice was higher after irradiation.

[0103] CuZn-SOD activity (U / ml) in the mouse serum of table 13

[0104]

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Abstract

The invention provides a method for establishing a radiation-induced vitiligo mouse model by applying different doses of Gamma-ray to carry out whole body irradiation of an IRM-2 mouse. The radiation-induced vitiligo mouse model is mainly used for the research of pathogenesis of vitiligo and the development of drugs for prevention and treatment, and can also be used for the research of tumourgenesis immune mechanism and the screening of targets for immunotherapy as well as for the research of radiation-induced immune dysfunction mechanism. The model of the invention is characterized by simple preparation, early lesion emergence time, high incidence, low radiation dose and so on, and further lays the foundation for the experimental research of the pathogenesis of vitiligo and the screening of new drugs at the same time.

Description

technical field [0001] The invention relates to a method for establishing a radiation-induced vitiligo mouse model, which is used for the research on the pathogenesis of vitiligo, the screening and evaluation of prevention and treatment drugs, and the research on the immune mechanism of tumor occurrence and the screening of immunotherapy targets. For research on the mechanism of radiation-induced immune dysfunction or radiation-regulated immune function. Background technique [0002] Vitiligo is a common depigmentation skin disease, clinically manifested as localized white spots on the skin, once onset, the cure rate is low and it is easy to protract, causing great physical and mental pressure to patients. The pathological change of vitiligo is mainly that the melanocytes that produce skin melanin are damaged. The cellular and molecular mechanisms leading to melanocyte destruction in vitiligo patients have not been elucidated. The etiology is very complex, and it is closel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B19/00A61K49/00A61D1/00
Inventor 王月英李德冠吴红英孟爱民路璐褚丽萍王彦张俊伶
Owner INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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