Innocent comprehensive approach method for human feces by biotechnology

A biotechnology and comprehensive treatment technology, applied in the cross field of biotechnology, chemical engineering technology and environmental protection technology, can solve the problems of spreading diseases, polluting the environment, lack of fertility, etc., achieve the development of energy saving and emission reduction, maintain and improve ecological balance , Significant social and economic benefits

Inactive Publication Date: 2008-11-19
昆明伽得科贸有限公司
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  • Abstract
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Problems solved by technology

[0006] The previous method of composting solid fermentation has been gradually eliminated due to the disadvantages of polluting the environment, spreading diseases, and lack of fertility.
At present, there is no mature and reliable treatment method in China

Method used

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  • Innocent comprehensive approach method for human feces by biotechnology

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Experimental program
Comparison scheme
Effect test

Embodiment

[0028] 1. Cultivation and operation of Bacillus subtilis

[0029] primary seed culture

[0030] Bacillus subtilis strains were purchased from Yunnan Institute of Biology. Inoculate the Bacillus subtilis species into the nutrient agar medium under aseptic conditions and cultivate it for 24-30 hours at a temperature of 37°C. Then add it into a sterile water test tube, shake well and vibrate for 30 minutes, and count with a hemocytometer after being uniform. When it reaches 108 cells / mL, the bacterial suspension can be obtained. If it is insufficient, the culture time should be extended.

[0031] Secondary value-added expansion training

[0032] Take out 1% of the Bacillus suspension. Put into the 500mL Erlenmeyer flask (inactivated) culture medium strain.

[0033] Medium formula: glucose 3%, peptone 1.5%, soybean juice 2%. Beef extract 3%, Tween 80 0.1%, dipotassium phosphine 0.2%, ammonium citrate 0.2%, ammonium sulfate 0.058%, sodium chloride 0.5%, water 400mL.

[0034]...

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Abstract

The invention relates to a method for realizing harmless comprehensive treatment of human excrements through adopting biotechnology, in particular to a comprehensive technology used for the harmless centralized treatment of human excrements in urban areas. The method comprises the following steps that: (1) human excrements, aquatic plants and terrestrial plants are added in a fermenter, and then a composite strain mixed solution of microzyme, bacillus and lactobacillus is fed into the fermenter so as to carry out aerobic fermentation and anaerobic fermentation in turn; (2) the biogas generated during fermentation is fed into a biogas generating set so as to generate electricity; (3) the products after fermentation undergoes solid-liquid separation, and the solid fermentation products are made into solid organic fertilizer through dewatering, drying, preparation and regranulation; (4) the liquid fermentation products is obtained after solid-liquid separation undergo decoloring, filtration and settlement layering, in the liquid, the sublayer liquid in the solution is made into liquid organic fertilizer through coarse filtration, secondary filtration, sterilization and preparation and the supernatant is made into rinsing meeting effluent standard through aeration and settlement filtration. The method can realize clean, environment-friendly and energy-saving ecological production.

Description

technical field [0001] The invention relates to a biotechnologically harmless comprehensive treatment method for human feces, which is a comprehensive technology for the harmless centralized treatment of urban human feces, and belongs to the intersecting field of biotechnology, chemical engineering technology and environmental protection technology. Background technique [0002] Existing feces treatment technology is the method of adopting solid fermentation. Feces are different from general urban domestic sewage, and feces contain a lot of sundries and suspended solids. Judging from the physical and chemical composition of feces, its pollution characteristics are high concentration of fecal residue, stench and pathogenic bacteria. The key to treating feces is how to effectively remove the organic matter and sundries in the feces, so as to eliminate the pollution of the feces to the environment and the harm to the human body. Removing the solidified matter in the feces and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C05F3/04B09B3/00C12R1/72C12R1/125C12R1/25
CPCY02E50/343Y02A40/20Y02E50/30Y02W30/40
Inventor 徐燕玲乔世伟徐贵元
Owner 昆明伽得科贸有限公司
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