In situ hybridization detection reagent box of RhoGDI2 gene, detection method and use thereof

A detection kit and in situ hybridization technology, applied in the field of kits, can solve the problems of tumor cell drug resistance, heterogeneity, failure in the anti-cancer war, etc., and achieve the effect of radically curing malignant diseases of cancer, convenient operation, and strong specificity

Active Publication Date: 2008-12-24
SUZHOU FUYING GENE TECH +2
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In 2005, the United States Institute of Health, Cancer Institute, Center for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer", that is to say, the mortality rate of cancer has not been reduced. Several factors for the failure of the cancer war are: 1. Heterogeneity of tumor cells; 2. Drug resistance of tumor cells; 3. Incomplete design of anticancer drugs, etc.
However, there is no report about the in situ hybridization detection kit and detection technology of RhoGDI2 gene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In situ hybridization detection reagent box of RhoGDI2 gene, detection method and use thereof
  • In situ hybridization detection reagent box of RhoGDI2 gene, detection method and use thereof
  • In situ hybridization detection reagent box of RhoGDI2 gene, detection method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A kind of in situ hybridization detection kit of RhoGDI2 gene

[0049] An in situ hybridization detection kit for RhoGDI2 gene, comprising a hybridization probe, a marker, a hybridization solution, and a synergist, wherein the sequence of the hybridization probe is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0050] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0051] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0052] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0053] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0054] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0055] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0056] Alkaline phosphatase antibody 1μl / tube 1 tube / box...

Embodiment 2

[0100] A kind of in situ hybridization detection method of RhoGDI2 gene

[0101] 1. Specimen processing:

[0102] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation medium, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation medium (blood: lymphocyte separation medium = 1:1.5), and centrifuge at 2000r / min 10min;

[0103] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0104] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0105] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospitals with conditions can use the film-ma...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an in situ hybridization detection kit of a RhoGDI2 (Rho GDP dissociation inhibitor beta) gene, a detection method and an application thereof. The kit comprises a hybridization probe, a label, a hybridization fluid and a potentiating agent, wherein, the hybridization probe sequence is shown in SEQ ID N0.1. The in situ hybridization detection method of the RhoGDI2 gene comprises the following steps that: a. the hybridization probe in the kit is contacted with the RNA to be detected in a zymolyte to form a hybridization complex; and b. the hybridization complex obtained in the step a is detected. The invention also relates to the application of the kit in the preparation of drugs for detecting the early cancer metastasis and recurrent diseases. The kit has the characteristics of high sensitivity and strong specificity. Meanwhile, the detection method has simple and convenient operation and can be used and popularized in the hospital over the district level.

Description

technical field [0001] The invention relates to a kit, in particular to a detection kit for in situ hybridization of RhoGDI2 gene and its detection method and application. Background technique [0002] According to the information provided by authoritative organizations at home and abroad, the number of new cancers in my country is 1.7 million every year, the death toll is nearly 1.6 million, and the number of patients is more than 6 million. In the world, there are 8 million new cancer patients every year, the number of deaths is close to 8 million, and the number of patients is about 84 million. Ten thousand people, the number will double by 2020, this is a set of terrible figures. [0003] In 2005, the United States Institute of Health, Cancer Institute, Center for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer", that is to say, the mortality rate of cancer has not been reduced. Several factors f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/53
CPCC12Q1/6886C12Q2543/10C12Q2563/131C12Q2600/118C12Q2600/136C12Q2600/112
Inventor 张云福裘建英
Owner SUZHOU FUYING GENE TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap