Compound frequently combined ultrasonic reinforced double-enzyme heparin sodium extracting technique

Abstract

The invention relates to a heparin sodium technique, in particular to a heparin sodium extracting technique utilizing multi-frequency combination and ultrasonic double-enzyme reinforcement. Firstly, the scraped small intestinal mucosa is sucked in a conventional enzymolysis vessel, and refined salt with a proportion of 2.3 percent to 2.8 percent by weight is firstly added thereto before heating, and the pH value is adjusted to 9.0 to 9.2; secondly, when the solution obtained from the first step is heated to 40 DEG C to 45 DEG C, trypsase and lipase are added, and the proportion is maintained between 10 : 0.5 to 10 : 5, the weight proportion of the double enzyme in the solution in the first step accounts for 0.3 percent to 0.4 percent, the solution is continuously heated to 50 DEG C to 55 DEG C, the enzymolysis under heat preservation is performed for 2 hours to 5 hours, then the solution is continuously heated to 80 DEG C to 85 DEG C, and the solution is filtered after being insulated for 10 minutes to 20 minutes; and thirdly, the filtered solution obtained from the second step is treated through the conventional steps of adsorbing, washing, eluting, precipitating, dehydrating and drying, so as to obtain the heparin sodium product. Compared with the prior art, the invention has the advantages that: firstly, the entire procedure time is substantially reduced by 33 percent; secondly, the yield of products is increased by 29.6 percent; and thirdly, the titer is stabilized to be higher than 120 (the titer of the domestic advanced technology product is between 90 and 110).

Description

Contents of the invention [0001] The present invention relates to a technology of heparin sodium, in particular to the background technology of the technology of double-enzyme extraction of heparin sodium combined with multiple frequency combination ultrasound. Background technique [0002] At present, almost all production in our country uses pig small intestine as raw material, and several methods such as salt hydrolysis, alkali hydrolysis and enzymatic hydrolysis are used in conventional reactors, among which salt hydrolysis is the most common application, and its production process is: raw material (intestinal mucosa), conventional reactor Inner brine extraction, filtration, resin adsorption, elution, ethanol precipitation, dehydration, drying, crude heparin sodium. [0003] Although the process of producing heparin sodium by the above method is relatively simple and easy to realize, the weak point of existence is that the process is delicate, because the crude product h...

AI Technical Summary

Problems solved by technology

[0003] Although the technological process of producing heparin sodium by the above-mentioned method is relatively simple and easy to realize, the weak point of existence is that the process is delicate, because the crude product heparin sodium is through repeated acid-base treatment, not only the yield of heparin sodium will be reduced, but also to the heparin sodium The biological activity is also greatly affected, especially the potency is difficult to reach more than 90USPu / mg, and the time is longer