Use of small intestine SP cells in preparing medicament for treating damage of intestines

An intestinal injury and small intestine technology, which is applied in the application field of small intestine SP cells in the preparation of drugs for treating intestinal injury, can solve the problems of limited material availability, inability to apply clinically, and difficulty in making breakthroughs in embryonic stem cells, and achieves improvement of chronic fibrosis and prolongation of survival. time, effect of good repair

Inactive Publication Date: 2009-02-25
何东南 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Stem cells are divided into different categories according to different stages of body development, such as embryonic stem cells, pluripotent stem cells, and committed stem cells in various tissues such as hematopoietic stem cells, neural stem cells, etc. According to the research results that have been obtained so far, embryonic stem cells are technically difficult to A breakthrough has been made, but it cannot be applied clinically in a short period of time. At the same time, it still faces many problems such as material limitations and ethical challenges.

Method used

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  • Use of small intestine SP cells in preparing medicament for treating damage of intestines
  • Use of small intestine SP cells in preparing medicament for treating damage of intestines
  • Use of small intestine SP cells in preparing medicament for treating damage of intestines

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Embodiment 1

[0035] Example 1, SP cell transplantation repairing radiation enteritis damage

[0036] 1. Preparation and phenotypic analysis of SP cells

[0037] In the present invention, in the absence of ideal small intestinal mucosal stem cell surface markers, combined with previous research results and the inventor's research results on the in vitro culture of small intestinal mucosal organoid fragments, it has been confirmed that small intestinal mucosal organoid fragments are preliminary. Based on the enriched stem / progenitor cell population, the small intestinal mucosa organoid fragment was used as the starting material, and the SP cells in it were isolated and further enriched. The specific method is as follows:

[0038] 1. Isolation of small intestinal mucosal organoid fragments

[0039] The isolation of murine intestinal mucosal organoid fragments is briefly described below. Take out the small intestine from the peritoneal cavity of ROSA26 mice (purchased from Jackson Laborator...

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Abstract

The invention discloses a new application of alvine SP cells, which is the application of alvine SP cells in the preparation of medicament for treating alvine injury. In the invention, alvine SP cells are in-situ implanted to a whole abdominal cavity exposed mouse, and the experiment proves that SP cells can return to alvine crypts to repair the injury, and also can prolong the surviving time of the animal model exposed by lethal dose, obviously improve the chronic fibrosis of radiation enteritis, and achieve nice repair effect on radiation alvine injury. The invention provides an innovative and convenient method and means for the clinic therapy of alvine injury diseases, and lays a foundation for seeking specific surface markings for stem cells of alvine mucous membrane.

Description

technical field [0001] The invention relates to a new application of small intestinal SP cells, in particular to the application of small intestinal SP cells in preparing medicine for treating intestinal injury. Background technique [0002] As the most important place for absorbing nutrients in the body tissues, the small intestine is one of the indispensable important organs in the body. At present, although most small bowel diseases can be effectively treated by various effective medical and surgical methods, there are considerable difficulties in the treatment of many refractory small bowel diseases, such as short bowel syndrome and postoperative radiotherapy for pelvic tumors. radiation enteritis of the small intestine. With the rapid development of enteral and parenteral nutritional support and the emergence of small bowel transplantation science, the treatment of these refractory small bowel diseases has made great progress. However, the development of things always...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/38A61P1/00
Inventor 何东南韩钦黎介寿赵春华
Owner 何东南
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