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Method for preparing ethanol from raw material containing cellulose

A technology of cellulose and cellulase, applied in the direction of biofuel, fermentation, etc., can solve the problem of low ethanol yield and achieve the effect of improving sugar conversion rate and yield

Active Publication Date: 2009-03-04
COFCO GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The purpose of the present invention is to overcome the defect that the ethanol production rate obtained by the existing method for preparing ethanol from cellulose-containing raw materials is relatively low, and provide a method for preparing ethanol using cellulose-containing raw materials with high ethanol production rate

Method used

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  • Method for preparing ethanol from raw material containing cellulose
  • Method for preparing ethanol from raw material containing cellulose
  • Method for preparing ethanol from raw material containing cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] This example is used to illustrate the method for preparing ethanol using cellulose-containing raw materials of the present invention.

[0062] (1) Steam explosion

[0063] test as figure 1 The shown corn stalk raw material, measured according to the above-mentioned method, the water content of the corn stalk is 20% by weight, and the raw material is cut into small sections of 1.5 cm × 0.2 cm × 0.5 cm. ) mixed with water whose weight is one third of the raw material weight of the corn stover and soaked to obtain corn stover with a water content of 40% by weight. Take 1000 grams of corn stover with a water content of 40% by weight, maintain the pressure at 195° C. for 5 minutes at a pressure of 1.8 MPa, and then release the pressure to complete steam explosion. The obtained steam-exploded product and water at 70°C were stirred and mixed for 30 minutes according to a weight ratio of 1:3, and then the solid-liquid was carried out at 900 rpm with a LW400 decanter centrifu...

Embodiment 2

[0082] This example is used to illustrate the method for preparing ethanol using cellulose-containing raw materials of the present invention.

[0083] (1) Steam explosion

[0084] Take 1000 grams of corn stalks with a moisture content of 50% by weight, cut them into small pieces of 1.3 cm × 0.4 cm × 0.8 cm, maintain at 190 ° C for 4 minutes at a pressure of 1.7 MPa, and then release the pressure to complete the steam explosion. A total of 1250 grams of steam exploded product (60 wt% water content).

[0085] According to the method for HPLC analysis of sulfuric acid hydrolyzed steam explosion products described in Example 1, it was found that 1250 grams of steam explosion products contained 213 grams of cellulose and 63 grams of hemicellulose in total.

[0086] (2) Enzymatic hydrolysis

[0087] According to the method of Example 1, the above-mentioned unwashed steam-exploded products were enzymatically hydrolyzed. The difference was that after adding water to the enzymatic hy...

Embodiment 3

[0090] This example is used to illustrate the method for preparing ethanol using cellulose-containing raw materials of the present invention.

[0091] (1) Steam explosion

[0092] Take 1000 grams of corn stover with a moisture content of 40% by weight, cut it into small pieces of 1.2 cm × 0.4 cm × 0.7 cm, maintain the pressure at 1.7 MPa at 200 ° C for 6 minutes, and then release the pressure to complete the steam explosion. The obtained steam explosion product and water at 75°C were stirred and mixed for 50 minutes in a weight ratio of 1:2.5, and then solidified at 900 rpm with a centrifugal pump LW400 decanter (Zhangjiagang Huada Centrifuge Manufacturing Co., Ltd.). After liquid separation, a total of 2000 grams of solid steam explosion products (water content of 70% by weight) were obtained.

[0093] According to the method for HPLC analysis of sulfuric acid hydrolyzed steam explosion products described in Example 1, it was measured that 260 grams of cellulose and 78 grams...

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Abstract

The invention relates to a method for preparing ethanol with a raw material containing cellulose; the method comprises the following steps: the ram material containing cellulose is blasted by steam; the obtained steam blasting product is mixed with enzyme to have zymohydrolysis; a zymohydrolysis product is obtained by fermentation, wherein, the method for mixing the steam blasting product and theenzyme comprises mixing the steam blasting product with the enzyme by at least two batches; the amount of each batch of steam blasting product added in is at most 60 percent of the weight of all the steam blasting product; the water content of the raw material containing cellulose is 30 weight percent to 60 weight percent. The yield of the ethanol prepared by the method is quite high.

Description

technical field [0001] The present invention relates to a method for preparing ethanol, and more particularly, to a method for preparing ethanol using cellulose-containing raw materials. Background technique [0002] The prior art method for preparing ethanol from cellulose-containing raw materials, such as straw, mainly includes three steps: 1. pretreatment of cellulose-containing raw materials, 2. enzymatic hydrolysis of cellulose, and 3. fermentation of sugar to produce ethanol. [0003] Cellulose-containing raw materials are generally plant roots, stems and leaves, including straw. The straw refers to the roots, stems and leaves left by crops (generally herbs) after harvesting fruits, mainly stems and leaves, such as corn straw, sorghum straw, cereal straw, and wheat straw. The main component of straw is lignocellulose. The lignocellulose is a mixture of cellulose, hemicellulose and lignin. The lignocellulose, which is formed by covalently or non-covalently bonding of...

Claims

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Application Information

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IPC IPC(8): C12P7/06
CPCY02E50/17Y02E50/10
Inventor 王国忠姜开荣谢成华孙长征岳国君林海龙邓立康
Owner COFCO GROUP