Ainsliaea fragrans Champ total flavone extract and extraction method and use thereof

A technology of apricot rabbit ear wind and extract, which is applied in the field of medicine, can solve the problems of outdated preparation process, large daily dosage, and low content of active ingredients, and achieve the effect of convenient operation, good repeatability, and simple process

Inactive Publication Date: 2009-03-11
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current commercially available rabbit ear wind preparations have outdated preparation methods, all of which are prepara

Method used

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  • Ainsliaea fragrans Champ total flavone extract and extraction method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Get 1 kg of rabbit ear wind medicinal material, pulverize, pass through a 20-mesh sieve, add 12000 ml of ethanol solution with a volume fraction of 5% prepared by a calcium hydroxide solution with a pH value of 9, reflux extraction for 2 hours, filter and save the filtrate, and repeat the operation for 3 Second-rate. The three filtrates were combined, the filtrate was concentrated to about 2 mg / ml of flavonoids, extracted three times with petroleum ether, and the aqueous layer was retained. The macroporous resin column that has been treated on the mother liquor (soaked in 95% ethanol for 12 hours, then eluted for 2-4 volumes with 95% ethanol, and then eluted with distilled water until the eluent has no alcohol smell), the flow rate is 1ml / min. Elute with a large amount of distilled water until the eluate is colorless, elute with 5 volumes of 10% ethanol, collect the eluate with 10% ethanol, concentrate and evaporate to dryness to obtain the purified rabbit ear wind, t...

Embodiment 2

[0016] Get 1 kg of rabbit ear wind medicinal material, pulverize, pass through a 20-mesh sieve, add 12000 ml of ethanol solution with a volume fraction of 10% prepared by a calcium hydroxide solution with a pH value of 8, reflux extraction for 2 hours, filter and save the filtrate, and repeat the operation for 3 Second-rate. The three filtrates were combined, the filtrate was concentrated to about 2 mg / ml of flavonoids, extracted three times with petroleum ether, and the aqueous layer was retained. The macroporous resin column that has been treated on the mother liquor (soaked in 95% ethanol for 12 hours, then eluted for 2-4 volumes with 95% ethanol, and then eluted with distilled water until the eluent has no alcohol smell), the flow rate is 1ml / min. Elute with a large amount of distilled water until the eluate is colorless, elute with 5 volumes of 30% ethanol, collect the eluate with 30% ethanol, concentrate and evaporate to dryness to obtain the purified rabbit ear wind, ...

Embodiment 3

[0018] Take 1 kg of rabbit ear wind medicinal material, pulverize, pass through a 20-mesh sieve, add 12000 ml of ammonia water with a pH value of 8 to prepare a 30% ethanol solution, reflux extraction for 2 hours, filter and save the filtrate and repeat the operation 3 times. The three filtrates were combined, the filtrate was concentrated to about 2 mg / ml of flavonoids, extracted three times with petroleum ether, and the aqueous layer was retained. The macroporous resin column that has been treated on the mother liquor (soaked in 95% ethanol for 12 hours, then eluted for 2-4 volumes with 95% ethanol, and then eluted with distilled water until the eluent has no alcohol smell), the flow rate is 1ml / min. Elute with a large amount of distilled water until the eluate is colorless, elute with 30% ethanol for 5 volumes, collect the eluate with 30% ethanol, concentrate and evaporate to dryness to obtain the purified rabbit ear wind, the total flavonoid content is about 69% .

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Abstract

The invention belongs to the pharmaceutical technology field and provides an extract of total flavonoids of frangrant ainsliaea herb, an extraction method thereof and a use. The extract adopts the frangrant ainsliaea herb as a raw material, ethanol which is prepared by weak alkaline solutions with the different pH values is taken as an extraction solvent, the reflux extraction is adopted, the impurities are removed by the petroleum ether extraction, the separation and the enrichment are finally carried by macroporous resin, and the purity of the components of the obtained total flavonoids is 50-95 percent. The invention has simple process, convenient and safe operation and good repeatability and can be used for the industrial production. The invention further provides an application of the components of the total flavonoids or a preparation which is prepared by the total flavonoids and a pharmaceutically acceptable carrier in the prepration of drugs for treating acute and chronic cervicitis.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to an extract of total flavonoids from apricot and rabbit ear wind, an extraction method and application thereof. Background technique [0002] Apricot-fragrance rabbit ear wind is a common folk medicine, which is the dried whole herb of Ainsliaea fragrans Champ. Apricot Fragrant Rabbit Ear Wind is sweet in nature and flavor, slightly bitter, cool in nature, and returns to the lung and liver channels. It has the effects of clearing away heat and invigorating deficiency, cooling blood for hemostasis, promoting dampness and detoxification. Indications for consumptive bone steaming, pulmonary rash and hemoptysis, women's uterine bleeding, damp-heat jaundice, edema, carbuncle and swelling, scrofula and tuberculosis, bruises, snake bites, etc. Clinically, Xingxiang rabbit ear wind preparation and folk prescriptions are used to treat cervical erosion, endometritis, hot boils, thrush and ...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P15/00
Inventor 王淑君邱宁周国勤杨瑞
Owner SHENYANG PHARMA UNIVERSITY
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