Immobilized lipase and preparation method thereof

Abstract

The invention discloses immobilized lipase and a method for preparing the same. The method for preparing the immobilized lipase comprises the following steps: evenly mixing lipase solution with polymer emulsion the weight of which is between 3 and 60 percent against that of the lipase solution, immersing a non-woven fabric by the obtained mixed liquid, and drying the mixed liquid to obtain the immobilized lipase, wherein the polymer emulsion is polyacrylate emulsion or the mixed emulsion containing polyacrylate and polyurethane as solutes. Experiments prove that the immobilized lipase has high catalysis performance and long service life, can still keep the catalysis efficiency above 90 percent after various continuous catalytic esterification reactions and transesterification reactions, and the required amount of the immobilized lipase for catalytically producing each ton of bio-diesel is less than or equal to 4 kilograms.

Description

A kind of immobilized lipase and preparation method thereof technical field The invention relates to an immobilized lipase and a preparation method thereof. Background technique The immobilization technology of biological enzymes is one of the core contents of enzyme engineering. It successfully overcomes the shortcomings of free enzymes in industrial applications, such as poor stability, poor heat resistance, non-recyclability and difficult separation from products. The stability and catalytic activity of the immobilized enzyme are greatly improved, and its reusability can greatly reduce the production cost. Enzyme immobilization technology can improve the microenvironment of enzyme molecular catalysis, facilitate the contact between enzyme and substrate and the diffusion of products. In addition, the immobilized carrier endows the immobilized enzyme with certain physical properties and mechanical properties, so that it can be conveniently applied to various bioreactors,...

AI Technical Summary

Problems solved by technology

This immobilized lipase has high activity, large enzyme load and long service life, but the process is complicated, the cost is high, the enzyme activity loss is serious (generally more than 60%) in the immobilization process, and the process has high requirements for the purity of the enzyme itself. high

The price of this kind of immobilized enzyme is very high, generally more than 1,000 yuan / kg, and some even exceed 10,000 yuan, which is not suitable for large-scale application in the market

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