Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting flavone substances in jumi

An extraction method and technology of flavonoids are applied in the field of extraction of flavonoids in chrysanthemum rice, which can solve problems such as unreported research, and achieve the effects of strengthening destruction, promoting release and leaching, and accelerating release.

Active Publication Date: 2011-06-22
PROYA COSMETICS
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, traditional organic solvents such as ethanol and methanol are used for extraction of natural active ingredients, such as heating reflux, extraction, etc.; however, there are no reports on the use of modern extraction methods such as ultrasonic and enzymatic methods to jointly prepare active ingredients in chrysanthemum.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1, a kind of extracting method of flavonoids in the rice chrysanthemum, carries out following steps successively:

[0014] 1) Weigh 20 g of chrysanthemum, add 200 mL of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a pH of 6.0, and add 3 g of cellulase (acid food-grade cellulase with an enzyme activity of 1000-1500 U / g) , 40°C water bath for 5h under stirring.

[0015] 2), add absolute ethanol to the resultant of step 1) to obtain a suspension, so that the volume concentration of ethanol in the suspension is 70%; the suspension is ultrasonically treated at a frequency of 150W for 0.5 hour, and suction filtered. The supernatant is obtained; the supernatant is vacuum-dried to obtain the chrysanthemum extract containing flavonoids. In the Jumi extract, the content of flavonoids is 28.0%.

Embodiment 2

[0016] Embodiment 2, a kind of extracting method of flavonoids in the rice chrysanthemum, carries out the following steps successively:

[0017] 1) Weigh 30g of chrysanthemum, add 240mL of acetic acid-sodium acetate buffer solution with a pH of 5.2, add 2.4g of cellulase (acid food grade cellulase, enzyme activity is 1000-1500U / g), stir 35 ℃ water bath for 6h.

[0018] 2), adding dehydrated ethanol to the resultant of step 1) to obtain a suspension, so that the volume concentration of ethanol in the suspension is 60%; the suspension is ultrasonically treated at a frequency of 200W for 1 hour, and suction filtered. The supernatant is obtained; the supernatant is vacuum-dried to obtain the chrysanthemum extract containing flavonoids. In the Jumi extract, the content of flavonoids is 28.3%.

Embodiment 3

[0019] Embodiment 3, a kind of extraction method of flavonoids in Ju Mi, carry out following steps successively:

[0020] 1) Weigh 20g of chrysanthemum, add 180mL of acetic acid-sodium acetate buffer solution with a pH of 4.6, add 3.6g of cellulase (neutral food grade cellulase, enzyme activity is 1000-1500U / g), 50°C Water bath for 5h.

[0021] 2), adding absolute ethanol to the resultant of step 1) to obtain a suspension, so that the volume concentration of ethanol in the suspension is 80%; the suspension is ultrasonically treated at a frequency of 180W for 0.5 hour, and suction filtered. The supernatant is obtained; the supernatant is vacuum-dried to obtain the chrysanthemum extract containing flavonoids. In the Jumi extract, the content of flavonoids is 26.5%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting a flavonoid substance from Jumi, which comprises the following steps in turn: firstly, the Jumi is weighed, added with a buffer solution of which the pH value is between 4.6 and 6.0, added with cellulase, and subjected to water bath for 5 to 6 hours at a temperature of between 35 and 50 DEG C; secondly, anhydrous alcohol is added into a substance obtained in the first step, and suspension is obtained; thirdly, the suspension is subjected to ultrasonic treatment and filtration to obtain supernatant; and fourthly, the supernatant is dried to obtain the Jumi extract containing the flavonoid substance. The method has the characteristics of simple and convenient operation, low production cost and good extraction effect.

Description

technical field [0001] The invention relates to a method for extracting flavonoids in Jumi. Background technique [0002] Jumi (Jumi) is very fragrant, round and bright yellow. It has the functions of detoxification, dispersing therapy, expelling wind, clearing fire and improving eyesight. It is produced in Suichang County, Zhejiang Province. It has a large yield and can be planted. It is mostly used for Beverages and traditional Chinese medicine compound are used in a very limited amount, which greatly limits the development of this characteristic industry. Chrysanthemum contains a variety of flavonoids, which can promote blood circulation in the human body and relieve spasm. At the same time, Chrysanthemum also has a variety of pharmacological and biological activities. It has antipyretic effect and has good curative effect on symptoms such as hypertension, migraine, coronary heart disease and angina pectoris. Using modern technology to efficiently and quickly extract fl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/28A61P9/10A61P9/12C12P17/06C12P19/60
Inventor 沈生荣于海宁阮晖姚超
Owner PROYA COSMETICS