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Test-tube breeding cultivation method of floral leaf Bambusa edulis

A technology of black-footed green bamboo and mosaic leaves, applied in the field of test-tube breeding and propagation of mosaic-leaved black-footed green bamboo, can solve the problems of adding bamboo families and achieve the effect of improving ornamental value

Inactive Publication Date: 2009-04-29
ZHEJIANG FORESTRY UNIVERSITY
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  • Abstract
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to use the method of test tube breeding to propagate the rare seedlings formed by this natural mutation, to expand the population as soon as possible, to breed a new variety of black-footed green bamboo with more ornamental value, and to add to the bamboo family. new member of

Method used

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Examples

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Embodiment Construction

[0009] The present invention will be described in further detail below in conjunction with the examples: In the culture medium for the test tube propagation of the sage green bamboo, the selected plant growth hormone is 3mg / LBA plus 1mg / L NAA, which is used after BA with 0, 0.3, 1, 3, 10mg / L plus 1mg / L NAA five kinds of concentration repeated 3 times, each time there are 20 cultures of each concentration of the comparative test, 3mg / L BA plus 1mg / L NAA treatment 75 After days, the buds grow the fastest and differentiate the most. In short, the culture medium and its ratio, temperature, light intensity and other culture conditions disclosed in the present invention are all preferred solutions. As carbohydrate nutrient bases, coagulants, etc., glucose, agar, etc. can also be substituted. It is obvious that the ratio can also be replaced by the close value of the best solution of the present invention. The best embodiment of the present invention is given below.

[0010] The test-tub...

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PUM

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Abstract

The invention relates to vitro breeding and reproduction of mosaic bambusa edulis, comprising steps of: a, choosing those vitro mosaic plantlets which have been through stable idiovariation as explants to subculture; b, root induction and transplanting, namely transplanting the plantlets to culture medium which is used for root induction and culturing for 60 days so as to reach 100% of rooting rate in normal, moving the plantlets to domestication room for two weeks and then transplanting in man-made substrate, planting the plantlets in greenhouse after one week. The invention propagates the species which is rare to find and stable in natural mutation and hereditary character, and it also cultivates novel species of mosaic bambusa edulis with good afforestation value, edible value and ornamental value.

Description

【Technical Field】 [0001] The invention relates to a method for in vitro breeding and breeding of new bamboo varieties through tissue culture technology. 【Background technique】 [0002] Black-footed green bamboo (Bambosa edulis) is a medium-sized clump bamboo belonging to the genus Poaceae Bamboo subfamily. It has high greening and ornamental value, especially as a bamboo shoot, with early shoots, high yield and good quality. The poles of black-footed green bamboo stand upright and are pale green, up to 20 meters high, with a thick base. The rod has many branches at each node, clustered in bunches, and the leaves are green. [0003] As early as April 19, 1990, it was accidentally discovered that there was a bud-turned seedling among the seedlings of black-footed green bamboo bred in test-tube. The 3-4 leaves at the base of the branch had white stripes. Select and keep this bud immediately. As a cultivating body, subculture in vitro and further transplant potted plants. It has been...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00C12N5/04
CPCY02A40/22Y02P60/40
Inventor 黄丽春方伟桂仁意林新春黄宝莲朱圆圆
Owner ZHEJIANG FORESTRY UNIVERSITY
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