Method for extracting xanthohumol from lupulus

A technology of xanthohumol and hops, which is applied in the field of xanthohumol and products containing the xanthohumol, can solve problems such as the lack of large-scale production, improve product yield and purity, simplify purification methods, and simplify processes Effect

Inactive Publication Date: 2009-05-27
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology uses certain chemical compounds from plants called betaxols (chemically related molecules) that have unique properties like being effective against bacteria or viruses without causing damage when ingested into humans. These chemistry allows these substances to effectively kill harmful microorganisms while reducing their impact upon human body's wellbeing. By combining this two different processes together, they improve both quality control measures such as extractive efficiency and ultraviolet light absorbance detection. Ultimately, this new technique makes production more efficient than current methods but still produces higher levels of impurities compared to previous techniques.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the quality of extracted beta hydrocarbons found within certain types of plant sources like luteks or worts while maintaining their stability during storage without decomposing themselves.

Method used

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  • Method for extracting xanthohumol from lupulus
  • Method for extracting xanthohumol from lupulus
  • Method for extracting xanthohumol from lupulus

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0033] The preparation of embodiment 1 xanthohumol

[0034] 1 Pretreatment of hops: After the hops are crushed, pass through a 60-mesh (0.30mm) sieve, and take out a part to measure the water content.

[0035] 2.1 Preparation of hops raffinate: the pulverized hops samples were subjected to supercritical CO under the following conditions 2 Extraction: extraction pressure is 20Mpa, extraction temperature is 45°C, CO 2 The flow rate is 25L / h, and the extraction time is 3h. Collect the raffinate and store it in a 4°C refrigerator for later use.

[0036] 2.2 Separation and extraction of xanthohumol: Weigh 75g of hop extract sample, add 750ml of organic solvent (methanol / ethanol), ultrasonically extract for 5min at a temperature of 20°C and a power of 300W, and then filter to remove the solid residue. Collect the filtrate for later use.

[0037] 2.3 Purification of xanthohumol:

[0038] a) Concentrate the collected filtrate at low temperature to 50ml with a rotary evaporator, a...

Embodiment 2

[0041] Example 2 Detection and Identification of Xanthohumol

[0042] 1. Ultraviolet scanning: After properly diluting the purified xanthohumol sample, scan it within the wavelength range of 150-500.

[0043] 2. LC-ESI-MS analysis and identification: Weigh a certain amount of purified xanthohumol sample, dissolve it in methanol solution according to a certain proportion, pass it through a 0.45um filter membrane, and wait for the test.

[0044] Liquid conditions: liquid chromatography: Agilent 1100; chromatographic column: Zorbax C18 (2.1×150mm, 5um); detector: DAD detector; wavelength: 370nm; column temperature: room temperature; flow rate: 0.2ml / min; injection volume : 30ul; mobile phase: methanol: water = 85:21

[0045] Mass Spectrometry Conditions:

[0046] Mass spectrometer: Thermoelectric Corporation (LCQ Deca XP);

[0047] Mass analysis instrument: ion trap;

[0048] Ion source: ESI;

[0049] Spray voltage: 4.5KV;

[0050] Capillary temperature: 300°C;

[0051] Sc...

Embodiment 3

[0063] Example 3 Determination of α-acid and β-acid

[0064] 1. Sample pretreatment

[0065] 1.1 Pretreatment of standard samples of hop extract: place standard samples of hop extract in a water bath at 25°C to 30°C, and stir well. Weigh 0.5g into a 50ml beaker, add 30ml methanol to dissolve, place in an ultrasonic water bath for 30min, transfer to a 100ml volumetric flask, dilute to volume with methanol, and mix well. Take 20ml in a 50ml volumetric flask, dilute to volume with methanol, and mix thoroughly. Filter with a 0.45um membrane, store in a sample bottle, and prepare for sample injection. Samples should be stored at low temperature and protected from light, so they are stable within 24 hours.

[0066] 1.2 Pretreatment of purified xanthohumol samples: Weigh 0.5g, add 30ml methanol to dissolve in a 50ml beaker, place in an ultrasonic water bath for 30min, transfer to a 100ml volumetric flask, dilute to volume with methanol, and mix well. Take 20ml in a 50ml volumetri...

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Abstract

The invention provides a method for extracting xanthohumol from hop and an obtained product thereof. The method mainly comprises the following steps: pulverized hop is extracted through supercritical carbon dioxide; a hop raffinate is collected and is subjected to ultrasonic extraction through methanol (ethanol) as an extractant; an extraction solution is condensed, is evenly mixed with diatomite and is successively eluted by two kinds of eluants; and the collected eluant is subjected to filtering and low-temperature condensation treatment to obtain the pure xanthohumol product. The purity of the obtained xanthohumol is 86.2 percent. The purified xanthohumol is a yellow powdery solid; and after the purified xanthohumol is recrystallized, the purity can reach over 97.8 percent. The method can achieve the functions of simplifying the xanthohumol extraction process and reducing the cost for purifying the xanthohumol. The obtained xanthohumol can be widely used in medicine, chemical engineering, food and other fields.

Description

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Claims

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Application Information

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Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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