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Method for rapidly screening oil refining wastewater bioflocculant-producing bacteria

A technology for microbial flocculants and oil refining wastewater, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve problems such as large workload, low screening efficiency, and uncertain screening targets.

Inactive Publication Date: 2009-05-27
BC P INC CHINA NAT PETROLEUM CORP +1
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AI Technical Summary

Problems solved by technology

The traditional method of screening microbial flocculant-producing bacteria is mainly to obtain pure cultures through plate separation, and then culture each pure culture in shake flasks to determine whether the fermentation broth has flocculation activity to determine flocculant-producing bacteria. There is a screening Uncertain target, heavy workload, long cycle, low screening efficiency and other disadvantages

Method used

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  • Method for rapidly screening oil refining wastewater bioflocculant-producing bacteria
  • Method for rapidly screening oil refining wastewater bioflocculant-producing bacteria
  • Method for rapidly screening oil refining wastewater bioflocculant-producing bacteria

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Embodiment 1

[0033] Screening of microbial flocculant-producing bacteria for oil refinery wastewater by pyridine screening

[0034] Take 2g of the sample and place it in a sterile mortar for grinding, then dilute it with sterile water to obtain a suitable concentration of bacterial suspension for later use. Use a 250mL Erlenmeyer flask to hold 60mL of enrichment medium H1, insert the numbered samples into it, and culture it on a shaker at 30°C and 140rpm for 48 hours, then transfer it to enrichment medium H2 with a 20% inoculum size, still 30 ℃, 140rpm shaker culture for 48 hours. Afterwards, 20% of the inoculum was added to the enrichment medium H3, and cultured on a shaker at 30° C. and 140 rpm for 48 hours. Dilute the enriched culture solution to obtain pure strains, and number them. Each single strain was inserted into the medium one by one, cultured with shaking at 28°C for 24 hours, and the culture solution was taken to measure its flocculation activity. (1) Determination method o...

Embodiment 2

[0051] Flocculation Test of Microbial Flocculants in Oil Refinery Wastewater Treatment

[0052] Thirteen strains with high flocculation rate were obtained from activated sludge by preliminary screening by pyridine screening method, and the flocculation experiment of refinery wastewater was further carried out. Add 300mL of oil refinery wastewater into a 500mL beaker, then add 50μL of microbial flocculant, and stir rapidly (200rpm) on a six-way mixer for 1min, so that the microbial flocculant and oil refinery wastewater are fully mixed to form fine alum flowers, and the wastewater is poured into the air pontoon. Use a small static air flotation device for air flotation. After standing for 5 minutes, take a 50mL water sample from the sampling port to measure the wastewater chemical oxygen demand (COD), petroleum and suspended solids, and calculate the removal rate of each indicator. The experimental results are shown in Table 6.

[0053] Table 6 The flocculation of oil refinery...

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Abstract

The invention discloses a method for rapidly screening a producing strain of a microbial flocculent of refinery wastewater, which can overcome the disadvantages of the prior screening method of the producing strain of the microbial flocculent, and can directionally screen producing strain of the microbial flocculent of the refinery wastewater according to the flocculent which needs to obtain corresponding application. The experiment shows that the average screening rate of the prior screening method is 26.6 percent, and the screening rate of the pyridine screening method is 44.3 percent. The method has the advantages of simple process, small workload, short period, and high screening efficiency.

Description

technical field [0001] The invention relates to a method for rapidly screening oil refinery wastewater microbial flocculant-producing bacteria, and the method belongs to the method optimization for rapid screening of flocculant-producing bacteria in the field of environmental protection. Mainly through specific screening procedures, the required strains are quickly selected to meet the actual needs of the site. Background technique [0002] The wastewater produced by petrochemical industry has complex components, large fluctuations in water quality and quantity, high concentration of pollutants and is difficult to degrade. Most of them are toxic and harmful organic substances, which seriously pollute the environment. With the increasing shortage of water resources and the strengthening of people's awareness of environmental protection, the treatment technology of petrochemical wastewater has gradually become a research hotspot. Flocculation is an important method for petroc...

Claims

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Application Information

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IPC IPC(8): C12N1/00C02F3/34C02F1/52
Inventor 赵东风张云波刘其友赵朝成
Owner BC P INC CHINA NAT PETROLEUM CORP
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