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Preparation of biological preservative 4-hydroxyphenyl lactic acid

A technology of hydroxyphenyllactic acid and hydroxyphenylpyruvic acid, which is applied in the preparation of biological preservative 4-hydroxyphenyllactic acid, and the field of microbial fermentation and biotransformation to prepare 4-hydroxyphenyllactic acid, can solve the problems of large individual differences of strains, low yield and the like

Inactive Publication Date: 2009-05-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has recently been reported that some lactic acid bacteria can use MRS medium to ferment 4-hydroxyphenyllactic acid, but the yield is low and the individual strains vary greatly. The highest yield is 0.29mmol / L

Method used

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  • Preparation of biological preservative 4-hydroxyphenyl lactic acid
  • Preparation of biological preservative 4-hydroxyphenyl lactic acid
  • Preparation of biological preservative 4-hydroxyphenyl lactic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 The influence of different strains on the output of 4-hydroxyphenyl lactic acid

[0022] Add 4-hydroxyphenylpyruvate of 4mmol / L in the fermentation medium, carry out the fermentation experiment to some lactic acid bacteria of primary selection according to the preferred medium mentioned above in the instructions, after 12 hours of fermentation, the fermentation liquid is detected, and the results are shown in Table 1 , all kinds of lactic acid bacteria can convert 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactic acid, among which Lactobacillus sp.SK007 has a higher 4-hydroxyphenyllactic acid fermentation yield, and different strains have a more significant effect on the production of 4-hydroxyphenyllactic acid difference.

[0023] Table 1 Effects of different strains on the production of 4-hydroxyphenyllactic acid

[0024]

Embodiment 2

[0025] Example 2 Effect of fermentation time on producing 4-hydroxyphenyl lactic acid

[0026] According to the fermentation conditions described in the manual, 4mmol / L 4-hydroxyphenylpyruvate was added, and the 4-hydroxyphenyllactic acid produced by Lactobacillus sp.SK007 at different fermentation times was detected, and the content of 4-hydroxyphenyllactic acid was found to be the highest at 18 hours of fermentation , with the extension of fermentation time, the content of 4-hydroxyphenyllactic acid decreased slightly. Therefore, the fermentation time was determined to be 18 hours, and the influence of the fermentation time on the production of 4-hydroxyphenyllactic acid was shown in Table 2.

[0027] Table 2 Effect of fermentation time on the production of 4-hydroxyphenyllactic acid

[0028]

Embodiment 3

[0029] Example 3 Effects of Different Substrates on the Production of 4-Hydroxyphenyllactic Acid

[0030] According to the fermentation conditions described in the manual, add 4mmol / L of different substrates 4-hydroxyphenylpyruvate or sodium 4-hydroxyphenylpyruvate respectively, and produce 4-hydroxyphenylpyruvate after 18 hours of fermentation by Lactobacillus sp.SK007 Detection, 4-hydroxyphenylpyruvate and sodium 4-hydroxyphenylpyruvate are close to the fermentation yield of 4-hydroxyphenyllactic acid as substrate, which proves that both 4-hydroxyphenylpyruvate and sodium 4-hydroxyphenylpyruvate are 4-hydroxyphenylpyruvate. Effective substrate for the fermentative production of hydroxyphenyllactic acid.

[0031] Table 3 Effect of different substrates on the production of 4-hydroxyphenyllactic acid

[0032]

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Abstract

The invention provides a method for a biological antiseptic 4-hydroxyl phenyllactic acid, which relates to a method for preparing 4-hydroxyl phenyllactic acid by fermentation bioconversion of microbe, and belongs to the field of food biotechnology. The preparation method comprises that lactic acid bacteria are used as fungi, 4-hydroxyl phenylypruvic acid or 4-hydroxyl phenylypruvic acid salt is used as a conversion substrate; and by adding a fermentation medium formed by carbon sources, nitrogen sources and inorganic slat, the 4-hydroxyl phenyllactic acid is prepared by fermentation bioconversion. The lactic acid bacteria are cultured at a temperature of between 25 and 40 DEG C in an MRS culture medium, grow in good conditions and are used as a seed culture solution; the fermentation medium carbon source is glucose, maltose, lactose or sucrose, and the nitrogen source is a mixture of one or more than one of corn steep liquor, yeast extract, peptone and soy bean pulp; and after fermentation, detections show that the concentration of the 4-hydroxyl phenyllactic acid in the fermentation liquid is between 1.0 and 20.0mmol / L. The product prepared by the method has the advantages of safety, reliability and strong bacteria inhibiting function.

Description

technical field [0001] The invention relates to a method for preparing a biological preservative 4-hydroxyphenyllactic acid, in particular to a method for preparing 4-hydroxyphenyllactic acid through microbial fermentation and biotransformation, and belongs to the field of food biotechnology. Background technique [0002] 4-Hydroxyphenyl lactic acid is a new antibacterial substance produced by lactic acid bacteria. Studies have shown that 4-hydroxyphenyl lactic acid has a broad-spectrum anti-fungal function. [0003] 4-Hydroxyphenyllactic acid is a natural preservative. It exists in natural honey and is non-toxic to human and animal cells. Lactic acid bacteria are generally recognized as safety (GRAS) microorganisms and have been used for food preservation for thousands of years. Therefore, 4-hydroxyphenyl lactic acid is considered to be a new type of food system that can be used in food systems in addition to the first-generation antibacterial substances such as lactic a...

Claims

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Application Information

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IPC IPC(8): C12P7/42C12R1/225C12R1/25
Inventor 沐万孟江波
Owner JIANGNAN UNIV
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