Method for preparing ciprofloxacin immunogen

Abstract

The invention discloses a method for preparing ciprofloxacin immunogen. The method for preparing ciprofloxacin immunogen of the invention is characterized by comprising the following steps: bovine serum albumin, ciprofloxacin, and 1-ethide-(3- dimethyl aminopropyl) carbodiimide hydrochloride are added into a phosphoric acid buffer liquid according to a proportion of 1 :503 :10433 for reaction for 2hours at the room temperature at first; then the liquid after reaction is put in a dialysis bag and put into 1L of the phosphoric acid buffer liquid to dialyze for 48 hours; the liquid is changed every 24 hours; finally the liquid in the dialysis bag is collected, pre-frozen at the temperature of -20 DEG C, then frozen and dried at the temperature of -50 DEG C to obtain the white solid powder of the ciprofloxacin immunogen. In the invention, the preparation process is simplified; the time for preparing the ciprofloxacin immunogen is shortened; the production efficiency is improved by about 37.5 percent; moreover, the production cost is reduced, thus being beneficial to popularization and application.

Description

Technical field: [0001] The utility model relates to a ciprofloxacin immunogen, in particular to a preparation method of the ciprofloxacin immunogen. Background technique: [0002] The preparation of ciprofloxacin immunogen usually needs to implement the process of synthesizing ciprofloxacin immunogen. However, in the process of synthesizing ciprofloxacin immunogen, existing methods generally carry out coupling synthesis in two steps, and the cost The time required is about 80 hours, which will prolong the process of synthesizing ciprofloxacin immunogen, resulting in reduced production efficiency and increased product cost. At the same time, the cost of subsequent use is also increased. In addition, its coupling ratio is generally only in the 2-6 or so. Invention content: [0003] The purpose of the present invention is to provide an improved preparation method of ciprofloxacin immunogen, which not only can effectively prepare ciprofloxacin immunogen, but also has simple ...

AI Technical Summary

Problems solved by technology

[0002] The preparation of ciprofloxacin immunogen usually needs to implement the process of synthesizing ciprofloxacin immunogen. However, in the process of synthesizing ciprofloxacin immunogen, existing methods generally carry out coupling synthesis in two steps, and the cost The time required is about 80 hours, which will prolong the process of synthesizing ciprofloxacin immunogen, resulting in reduced production efficiency and increased product cost. At the same time, the cost of subsequent use is also increased. In addition, its coupling ratio is generally only in the 2-6 or so