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Biotoxin detecting method

A detection method and biotoxicity technology, applied in the field of biotoxicity detection, can solve the problems of few species of luminescent bacteria, limited living environment, incompatibility, etc., and achieve the effects of improving the scope of application, improving the detection sensitivity, and expanding the selection range.

Active Publication Date: 2009-06-10
FOCUSED PHOTONICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few types of luminescent bacteria and their living environment is limited. If only luminescent bacteria are used as the only marker for environmental biological toxicity detection, it cannot meet the needs of various complex environments.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A detection method for biological toxicity, comprising the following steps:

[0039] a. Selection steps

[0040] According to the needs of detecting the samples to be tested, select Bacillus subtilis, which contains fluorescent substances, such as riboflavin, reduced nicotinamide adenine dinucleotide and adenosine triphosphate;

[0041] The culture medium commonly used by Bacillus subtilis is used for cultivation, and when it is cultivated to the logarithmic growth phase, a bacterial suspension of appropriate concentration is prepared and added to the test tube;

[0042] b. Mixing step

[0043] Add the liquid to be tested into the test tube, such as a solution containing mercuric chloride, the sample to be tested, to make it fully contact with the bacteria;

[0044] c. Detection steps

[0045] The light source emits measurement light, the wavelength of the measurement light includes 270nm, 292nm and 344nm, and the measurement light reaches the mixed liquid;

[0046]...

Embodiment 2

[0051] A detection method of biological toxicity, different from Example 1 is:

[0052] Step a is:

[0053] According to the needs of detecting the samples to be tested, select Bacillus subtilis, which contains fluorescent substances, such as riboflavin, reduced nicotinamide adenine dinucleotide and adenosine triphosphate;

[0054] The culture medium commonly used by Bacillus subtilis is used for cultivation, and after being cultivated to the logarithmic growth phase, the freeze-dried powder is made by freeze-drying method;

[0055] The solution is added to the freeze-dried powder to restore the Bacillus subtilis to its original physiological state, and the bacterial suspension prepared as an appropriate concentration is added to the test tube.

Embodiment 3

[0057] A detection method of biological toxicity, different from Example 2 is:

[0058] Step c is:

[0059] The light source emits measurement light, the wavelength of the measurement light includes 270nm, 292nm and 344nm, and the measurement light reaches the mixed liquid;

[0060] Among them, 270nm light excites riboflavin in Bacillus subtilis to emit fluorescence, 292nm light excites adenosine triphosphate in Bacillus subtilis to emit fluorescence, and 344nm light excites reduced nicotinamide adenine dinucleotide in Bacillus subtilis to emit fluorescence;

[0061]The fluorescence emitted by riboflavin was detected at 518 nm, the fluorescence emitted by adenosine triphosphate was detected at 388 nm, and the fluorescence emitted by reduced nicotinamide adenine dinucleotide was detected at 465 nm;

[0062] The fluorescence measurement was kept for 5 minutes to obtain the dynamic data of the reaction; according to the dynamic change of the fluorescence, the biological toxicit...

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Abstract

The invention discloses a detecting method of biotoxicity, comprising: a. a selecting step, selecting animalcule including fluorescent materials; b. a mixing step, adding liquid to be detected to the animalcule and mixing, wherein the liquid to be detected comprises samples to be detected; c. a detecting step, the light emitted by the light source reaches the mixed liquid, inspires the fluorescent materials to emit fluorescence; measuring fluorescence emitted by the fluorescent materials; analyzing change of the fluorescence, obtaining biotoxicity of the sample to be detected. The invention has the advantages of wide application range.

Description

technical field [0001] The invention relates to the detection of biological toxicity of various samples in the environment, in particular to a detection method of biological toxicity. Background technique [0002] Human beings live in the environment, and some components in the environment may cause harm to human life and health, so it is necessary to detect and monitor the environment. However, traditional physical and chemical analysis methods can only perform single detection of certain specific physical and chemical indicators such as temperature, pH value, dissolved oxygen, etc., and the impact of various substances in the environment on the human body is a comprehensive synergistic effect. Therefore, it is necessary to test the combined effects of these substances, and biological toxicity testing is such a technology. [0003] At present, the methods used for biological toxicity detection are: detection method based on bacterial growth inhibition; detection method bas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C12Q1/18
Inventor 吴坚
Owner FOCUSED PHOTONICS
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