Graft and rapid propagation method for commercial production of Paeonia rockii

A technology of purple-spotted peony and peony, which is applied in botany equipment and methods, horticulture, application, etc., can solve the problems of inconvenient management and maintenance, failure to meet the requirements of commercial seedlings, and long period of emergence from the nursery, so as to ensure accuracy and stability, Improve the efficiency of scale production and solve the effect of variety confusion

Inactive Publication Date: 2009-07-08

兰州牡丹园艺开发公司

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AI-Extracted Technical Summary

Problems solved by technology

The propagation speed of split root method is very slow, the seedlings produced are uneven, the number of branches is different, the root system is large and small, and the age of seedlings cannot be defined, which cannot meet the requirements of commercial seedlings, which affects the commercial production of purple spot peony. quality

Although the sowing method can increase the number of reproduction, but because the stamens and stamens of the better varieties of Gansu peony degenerate, the amount of seeds set is small, and the single-petal varieties with poor quality have a large amount of seeds, which makes the more degenerate the variety after sowing and reproduction, and it is impossible to make high-quality peonies. A large number of species and rare species have been multiplied, and it cannot meet people's viewing needs for fine varieties of peonies in Gansu

[000...

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Method used

(5) scion is collected: gather scion by kind color separation system, select diameter 0.7~1.1cm terminal bud and axillary bud are plump in that year's raw branch, the branch that gathers packs shade immediately and preserves, strictly prevents exposure to the sun, avoids branch dehydration; The harvested branches are cut into scions with a length of 8-10 cm. It must be ensured that each scion has 1-2 full buds. The collected scions must be grafted within 24 hours after disinfecti...

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Abstract

The invention discloses a rapid grafting propagation method for commercial production of paeonia rockii, and relates to the fields of grafting and propagation of flowers. The method comprises the following steps: establishing a fine variety scion collecting garden, establishing a rootstock production garden, preparing before grafting, selecting rootstocks, collecting scions, sterilizing the scions, sampling inspection, grafting, storing in sand, managing the sand storage, taking out of a greenhouse, planting and the like. The method helps determine a principle for selecting a grafting time based on temperature, thus improving the survival rate of the rare species (the paeonia rockii) from original 35% to more than 75%, causing large-scale propagation of the paeonia rockii to be possible, and providing a technical guarantee for the commercial production. The method helps realize the desire that the paeonia rockii in Gansu blossoms in any cold areas, and also meets the requirements of people from different areas for admiring rare species.

Application Domain

Horticulture

Technology Topic

Rare speciesCold area +8

Examples

Experimental program(2)

Example Embodiment

[0039] Example 1. In this embodiment, peony root is used as the rootstock. A method for commercialized production of purple spotted peony grafting and rapid propagation, including the following steps:

[0040] (1) Establish a good variety scion collection nursery: In the scion collection nursery, excellent Gansu purple-spotted peony varieties are planted and planted according to the color system. The plant row spacing is 2m×2m, and there are more than 100 varieties. Each plant is numbered and listed for registration. On average, the ear-picking nurseries are inspected every 15 days, the plants with pests and diseases are sprayed in time, and the severe ones are immediately dug out and destroyed to prevent infection. Apply farmyard manure once every October to the whole nursery at a rate of 1500 kg/mu, and apply topdressing once in March with a rate of 20 kg/mu. Prune regularly to ensure a healthy female parent.

[0041] (2) Establish a peony rootstock production nursery: The rootstock nursery plant peony seedlings, which can be used as rootstocks 2 to 3 years after sowing, or the lateral roots of qualified perennial seedlings can be selected as rootstocks.

[0042] (3) Preparation before grafting:

[0043] ① Grafting workshop: Seal the workshop with aluminum phosphide and fumigate for 12 hours before grafting to completely kill germs. During the grafting period, the workshop was sprayed and disinfected with 50% chlorothalonil 1500 times solution every morning and evening, and the residual branches and roots of the rootstock and scion were cleaned out in time.

[0044] ② Grafting workbench: the workbench is 60cm high, 2m wide, 15-20m long, and the average workbench area per person is 2m 2.

[0045] ③Personnel training: The grafting personnel must undergo strict training and can only work after passing the assessment. They must wear gloves when grafting, and the grafting tools must be disinfected regularly.

[0046] (4) Selection of peony rootstocks: Choose the main roots with a diameter of 0.8~1.2mm, which are strong and grow free from diseases and insect pests, with a length of 25~35cm, and cut off the germination point of the stock.

[0047] (5) Scion collection: Collect the scion according to the color separation system of the species, select the current-year-old branches with a diameter of 0.7-1.1cm, which are full of top and axillary buds. The collected branches are immediately packed in boxes and shaded to prevent exposure to the sun and avoid dehydration of the branches. Cut the harvested branches into scions with a length of 8-10cm, and ensure that each scion has 1 to 2 full buds. The harvested scions must be grafted within 24 hours after disinfection.

[0048](6) Disinfection method of scion:

[0049] ①50% chlorothalonil 800~1000 times solution soaked for 25~30 minutes; or

[0050] ②Soak in 800-1000 times solution of 75% thiophanate methyl for 20-25 minutes; after soaking, dry it in the shade for 1 to 2 hours until the scion epidermis is dry and then grafting can be carried out.

[0051] (7) Sampling inspection: 2% of the rootstocks and scions are randomly selected for inspection every day, and more than 95% of the rootstocks and scions can be grafted.

[0052] (8) Grafting: The grafting condition is to choose the temperature of 20~25℃, the ground temperature of 19~22℃, and the choice of grafting time is determined according to the temperature. When working, flatten the top of the root anvil and cut it diagonally. Then cut the scion with one bud and cut it slightly shorter than the cut of the root anvil. Align the cut of the root anvil with the scion and make close contact. The upper surface of the scion is 2mm. Leave it blank and tie it tightly with twine. When grafting, one person has one variety. After the grafting is completed, the brand is listed to ensure the accuracy of the variety. A designated person is responsible for classifying and counting the quantity and spot-checking the quality.

[0053] (9) Sand storage: The grafted seedlings must be stored in sand within 4 hours to prevent dehydration. Before sand storage, mix the coarse sand: fine sand according to 3:1, disinfect and mix the sand and store it wet. The humidity of the sand is suitable for the hands to hold water; after the sand is stored, cover the grass curtain to keep it moisturizing. Sand disinfection methods are as follows:

[0054] 75% Dixon 800-1000 times liquid spray disinfection; or

[0055] Spray disinfection with 50% thiophanate methyl 800-1000 times solution.

[0056] If the outside temperature is 20-25℃ during the grafting period and the ground temperature is 19-22℃, it can also be planted directly. Not only the survival rate can reach 60-65%, but also the sand storage and other subsequent steps are omitted.

[0057] (10) Sand storage management and protection: After sand storage, the sand bed should be managed and protected, especially the temperature and humidity control. The temperature, hygrometer and ground thermometer must be placed in the shed to keep the humidity at 65-75%. The temperature during the day is 25~28℃, and the temperature at night is 15~18℃. When the temperature is high during the day, pay attention to ventilation and cooling. Observe 4 times a day, record the temperature, humidity, and ground temperature, and spray water in time to keep the grass curtain moist, and spray water evenly. In order to prevent the inadequate spray water and the sand bed from being too dry, the grafted seedlings will be dehydrated by air drying.

[0058] Observe the healing of grafted seedlings about 15 days after sand storage, and the ones that produced callus were qualified seedlings.

[0059] Peony root grafted seedlings are stored in sand for 35-40 days.

[0060] (11) Planting out of the shed.

Example Embodiment

[0061] Example 2. In this embodiment, peony root is used as the rootstock. A method for commercialized production of purple spotted peony grafting and rapid propagation, including the following steps:

[0062] (1) Establish a good variety scion collection nursery: In the scion collection nursery, excellent Gansu purple-spotted peony varieties are planted. They are classified and planted according to the color system. The row spacing is 2m×2m, and there are more than 100 varieties. Each plant is numbered and listed for registration. On average, the ear-picking nurseries are inspected every 15 days. Plants with plant diseases and insect pests are sprayed in time, and the severe ones are immediately dug out and destroyed to prevent infection. Apply farmyard manure once in the whole nursery in October every year, with a fertilizer rate of 1500 kg/mu, and apply topdressing once in March with a fertilizer rate of 20 kg/mu. Prune regularly to ensure that the female parent is healthy.

[0063] (2) Establish a production nursery for rootstocks of peony rootstocks: plant the single-petal species Fenyunu with low ornamental value, and carefully manage them after sowing. They can be used as rootstocks within 2 to 3 years. Perennial peony rootstocks can also be dug to obtain rootstocks that meet the requirements.

[0064] (3) Preparation before grafting:

[0065] ① Grafting workshop: Before grafting, the workshop shall be sealed and fumigated with aluminum phosphide for 12 hours to completely kill the germs. During the grafting period, the workshop was sprayed and disinfected with 50% chlorothalonil 1500 times solution every morning and evening, and the residual branches and roots of the rootstock and scion were cleaned up in time.

[0066] ② Grafting workbench: The workbench is 60cm high, 2m wide, 15-20m long, and the average workbench area per person is 2m2.

[0067] ③Personnel training: The grafting personnel must undergo strict training and can only work after passing the examination. They must wear gloves when grafting, and the grafting tools must be disinfected regularly.

[0068] (4) Selection of peony rootstocks: smooth skin, no pests and diseases, diameter 1.0~2.5mm, length 10~15cm.

[0069] (5) Scion collection: Collect the scion according to the color separation system of the species, and select the current-year-old branches with a diameter of 0.7-1.1cm in the top and axillary buds. The collected branches are immediately packed in a box and shaded to prevent exposure to the sun and avoid dehydration of the branches; Cut the branches into scions with a length of 8-10cm. It must be ensured that each scion has 1 to 2 full buds. The harvested scions must be grafted within 24 hours after disinfection.

[0070] (6) Disinfection method of scion:

[0071] ①50% chlorothalonil 800~1000 times solution soaked for 25~30 minutes; or

[0072] ②Soak in 800-1000 times solution of 75% thiophanate methyl for 20-25 minutes; after soaking, dry it in the shade for 1 to 2 hours until the scion epidermis is dry and then grafting can be carried out.

[0073] (7) Sampling inspection: 2% of the rootstocks and scions are randomly selected for inspection every day, and more than 95% of the rootstocks and scions can be grafted.

[0074] (8) Grafting: The grafting condition is to choose the temperature of 20~25℃, the ground temperature of 19~22℃, and the choice of grafting time is determined according to the temperature. When working, flatten the top of the root anvil and cut it diagonally. Then cut the scion with one bud and cut it slightly shorter than the cut of the root anvil. Align the cut of the root anvil with the scion and make close contact. The upper surface of the scion is 2mm. Leave it blank and tie it tightly with hemp rope; when grafting, there is one species per person. After the grafting, the card will be listed to ensure the accuracy of the species. A dedicated person is responsible for classifying the quantity and spot-checking the quality.

[0075] (9) Sand storage: the grafted seedlings must be stored in sand within 4 hours to prevent dehydration; before sand storage, mix coarse sand: fine sand according to 3:1, disinfect and mix wet sand and store it in the sand. The humidity of the sand is the hand grip. It is advisable to keep the water out; cover the sand with a straw curtain to keep it moisturizing. Sand disinfection methods are as follows:

[0076] 75% Dixon 800-1000 times liquid spray disinfection; or

[0077] Spray disinfection with 50% thiophanate methyl 800-1000 times solution;

[0078] If the outside temperature is 20-25℃ during the grafting period and the ground temperature is 19-22℃, it can also be planted directly. Not only the survival rate can reach 60-65%, but also the sand storage and other subsequent steps are omitted.

[0079] (10) Sand storage management and protection: After sand storage, the sand bed should be managed and protected, especially the temperature and humidity control. The temperature, hygrometer and ground thermometer must be placed in the shed to keep the humidity at 65-75%. The temperature during the day is 25~28℃, and the temperature at night is 15~18℃. When the temperature is high during the day, pay attention to ventilation and cooling. Observe 4 times a day, record the temperature, humidity, and ground temperature, and spray water in time to keep the grass curtain moist. Rot, therefore, spray the water evenly to prevent the flow from entering the sand bed and cause rotten roots. Since the root of peony root is fleshy and healed earlier, the healing of grafted seedlings was observed about 13 days after sand storage, and the seedlings that produced callus were qualified seedlings.

[0080] The peony root grafted seedlings are stored in sand for 30 to 35 days.

[0081] (11) Planting out of the shed.

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PUM

Property	Measurement	Unit
Diameter	1.0 ~ 2.5	mm
Length	10.0 ~ 15.0	cm
tensile		MPa
Particle size		Pa
strength	10

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