Method for breeding haploidy Potherb mustard

It is a technology of menu and cultivation, which is applied in horticultural methods, botany equipment and methods, and plant gene improvement, etc. It can solve the problem of low frequency of embryos in the suspension culture of pickled cabbage microspores, and achieve the goal of improving the survival rate of embryos and increasing the frequency of occurrence Effect

Inactive Publication Date: 2009-07-08
ZHEJIANG UNIV
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Problems solved by technology

[0005] The invention provides a method for breeding pectin ploidy, and the method solves the problem of low frequency of embryonic occurrence in suspension cultured microspores of prickly ash

Method used

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Embodiment Construction

[0015] culture medium

[0016] NLN-13 medium: Take the configuration of 1L culture medium as an example, 100ml of macroelements (solution), 10ml of trace elements (solution), 10ml of organic matter (solution), 10ml of folic acid (solution), 10ml of Fe salt (solution), 10ml Inositol (solution), 130g sucrose, 30mg glutathione, 800mg L-glutamine, 100mg serine were mixed, the final pH was adjusted to 6.0, filtered and sterilized and placed in a 4°C refrigerator for later use.

[0017] Wherein macroelement (solution), microelement (solution), organic matter (solution), Fe salt (solution), folic acid (solution), inositol (solution) each composition ratio is as follows:

[0018] I. Macroelement (solution) (g / L) III. Organic matter (solution) (g / L) KNO 3 1.25 Vitamin B1 0.05 Ca(NO 3 ) 2 4H 2 o 5 Vitamin B6 0.05 MgSO 4 ·7H 2 o 1.25 Vitamin B5 0.5 K H 2 PO 4 1.25 Biotin 0.005 II. Trace elements (solution) (g / L) Glyc...

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Abstract

The invention discloses a potherb mustard haploid breeding method. The method comprises the following: taking an inflorescence on a main stem or a big branch of potherb mustard, and selecting and sterilizing buds from a late monokaryon stage to an early dikaryon stage after low temperature induction; placing the sterilized buds into NLN-13 culture solution, mashing, filtering and centrifuging to obtain a microspore suspension; performing a heat shock treatment on the microspore suspension and then culturing the microspores at the normal temperature in the dark to obtain embryoids in a cotyledon period; collecting and transferring the embryoids in the cotyledon period to a solid differentiation medium for differentiation culture, and transplanting seedlings into nutritional soil after rooting and hardening to obtain haploid plants; and treating root systems of the haploid plants with double colchicine to obtain dihaploid plants. In an embryoid induction process, the buds are subject to the low-temperature induction and the microspores are subject to the heat shock treatment, which improves embryoid production frequency and embryoid survival rate up to 95%.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for breeding snow diploids. Background technique [0002] Pickled cabbage is a mustard seed of the genus Brassica in the family Brassicaceae, and it is a variety of tillering mustard. There are many aliases for pickled cabbage, such as "Potherb mustard" and "Nine head mustard" in Anhui, Jiangsu and Zhejiang, and "Paicai" in Hunan, Hubei and other places. It is an important vegetable in winter and spring that is widely cultivated in the Yangtze River Basin in my country. It uses petioles and leaves as edible parts and has high nutritional value. The rich aroma, crisp and delicious Dongpo pork, shredded pork noodles with pickled vegetables, winter bamboo shoots with pickled vegetables, tofu with pickled vegetables, and yellow croaker with pickled vegetables are cooked with pickled vegetables as the main ingredients. [0003] The so-called haploid breeding refers t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00
Inventor 万光龙周伟军王淑珍许玲马华升
Owner ZHEJIANG UNIV
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