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Method for cultivating aseptic seedling of Ferula sinkiangensis

A cultivation method and technology for sterile seedlings, applied in germination equipment, seed immunization, seed and rhizome treatment, etc., can solve the problems of inability to form large-scale, harsh growth environment, and reduced success rate of cell culture, so as to maintain biodiversity. , Improve the success rate of training and protect the effect of drug resources

Inactive Publication Date: 2009-07-15
新疆维吾尔自治区中药民族药研究所
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the ferulicum plant resources in Xinjiang are in a state of danger, and it is difficult to meet the growing market demand of traditional Chinese medicine and ethnic medicine. Therefore, it is very urgent to carry out artificial rapid propagation and resource protection.
[0003] Asafoetida in Xinjiang is a short-lived plant in early spring. It grows rapidly. In addition to the harsh growth environment, the tissue is easy to age. It is difficult to grasp the best time to collect culture materials. Therefore, the success rate of cell culture is greatly reduced. The methods and means currently used are limited to early spring culture. The seedling raising work of Ferulicum is limited, and it cannot be scaled up. In particular, the seeds of Ferulicum ferulae in Xinjiang have physiological dormancy phenomenon in the natural environment. The germination rate and success rate of seedlings are very low in the usual method, and it is especially difficult to cultivate aseptic seedlings. The use of indoor cultivation and breeding of aseptic seedlings of Xinjiang ferulae can not only provide seedlings throughout the year, but also ensure that there are relatively tender cultured tissues and avoid direct damage to young tissues by disinfectants, improve the success rate of cultivation, and then can Effectively protect wild medicinal resources in Xinjiang, maintain biodiversity, and provide stable and sustainable medicinal resources for the pharmaceutical industry, especially the market demand for traditional Chinese medicine and ethnic medicine

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0018] Select clean, plump, and consistent seeds, immerse the selected seeds in 70% alcohol for 25 seconds, drain until there is no dripping, and then place them in 10% H2O by volume. 2 o 2 Soak in the solution for 15 minutes, drain until there is no drip, rinse with sterile water, and drain until there is no drip. After draining until there is no drip, rinse twice with sterile water twice the weight of the seed, and drain until there is no drip. The germination bed is a sand bed, and the sand bed is first cooled to 4-10°C, the detoxified seeds are placed on the seed germination bed, and cultivated at 4°C for 30 days. During the germination period, observe and record the germination situation at any time. When the radicle breaks through more than 1 / 2 of the length of the seed, it can be As a material for tissue culture, seedling planting or transplanting in the next step.

Embodiment 2

[0020] Select clean, plump, and consistent seeds, immerse the selected seeds in 50% alcohol for 35 seconds, drain until there is no dripping phenomenon, and then place them in H2O with a concentration of 15% by volume. 2 o 2 Soak in the solution for 10 minutes, drain until there is no dripping phenomenon, rinse with 5 times the weight of sterile water 3, drain until there is no dripping, spread the sterilized seeds on the sterile germination bed on the fully sterilized sand bed , the thickness should be such that there is no upper and lower overlap between the seeds. Keep the relative humidity of the environment at 55%, and cultivate it at a temperature of 10°C for 60 days. During the germination period, observe and record the germination situation. When more than 70% of the seed radicle breaks through more than 1 / 2 of the seed The resulting germ can be used as a material for tissue culture, seedling planting or transplanting and cutting in the next step.

Embodiment 3

[0022] Select clean, plump, and consistent seeds, immerse the selected seeds in 60% alcohol for 30 seconds, drain until there is no dripping, and then put them in H2O with a volume percentage concentration of 14%. 2 o 2 Soak in the solution for 14 minutes, drain until there is no drip, rinse with sterile water, and drain until there is no drip. Rinse twice with sterile water 3 times the weight of the seeds until there is no dripping, and drain until there is no dripping. The germination bed is a sand bed, and the sand bed is first sterilized by ultraviolet rays for 60 minutes, and the seeds after decontamination Place it on the seed germination bed and culture it at 7°C for 30 days. During the germination period, observe and record the germination situation at any time. When the radicle of more than half of the seeds breaks through more than 1 / 2 of the length of the seeds, it can be used as the next step Materials for tissue culture, seedling planting or transplanting, etc. ...

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Abstract

The invention discloses a ferula seedling-raising method, in particulate relates to a ferula sinkiangensis aseptic seedling cultivation method which is realized mainly through the procedures of seed selection, disinfection and germination bed culture. Compared with the prior art, the invention has the advantages that the method breakthroughs the lamination of the traditional ferula seedling-raising method, greatly improves the sprouting rate of seeds, can realize the scale ferula seedling-raising, can especially cultivate the ferula sinkiangensis aseptic seedlings indoors, can provide the seedlings all the year round, can ensure the culture tissues younger and avoid the direct harm of the disinfectant to the young tissues, improves the success ratio, realizes the scale tissue culture rapid propagation system of the ferula sinkiangensis, effectively protects Sinkiang wild medical resources, maintains the diversity of organisms, provides stable and sustainable medicinal resources for market demands of the pharmaceuticals industry, especially for the Chinese traditional medicine and the ethnic medicines, and has significant value.

Description

Technical field: [0001] The invention relates to a method for growing seedlings of Asafoetida, in particular to a method for cultivating aseptic seedlings of Asafoetida in Xinjiang. technical background: [0002] Ferula sinkiangensis K.M.Shen is a perennial early-spring short-lived plant of the genus Ferula L. in the Umbelliferae family. It is recorded in the "Pharmacopoeia of the People's Republic of China" in previous editions and has extremely high medicinal value. However, the plant resources of ferulicum in Xinjiang are in a state of danger, and it is difficult to meet the growing market demand of traditional Chinese medicine and ethnic medicine. Therefore, it is very urgent to carry out artificial rapid propagation and resource protection. [0003] Asafoetida in Xinjiang is a short-lived plant in early spring. It grows rapidly. In addition to the harsh growth environment, the tissue is easy to age. It is difficult to grasp the best time to collect culture materials. T...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01C1/02A01C1/08
Inventor 李晓瑾朱军凯撒·苏莱曼贾晓光梁明贾新岳石明辉王果平阿依别克·热河木都拉姜林
Owner 新疆维吾尔自治区中药民族药研究所
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