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Use of methionine

A technology of methionine and usage, applied in the field of medicine, can solve problems such as cochlear and vestibular toxic damage, vestibular dysfunction, etc.

Inactive Publication Date: 2012-12-12
曲雁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Many drugs and chemical agents are ototoxic and can also cause toxic damage to the cochlea and vestibule, resulting in deafness and / or vestibular dysfunction

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The characteristic of hair cell damage in deafness-enzyme and cytochemical reaction in hair cells

[0061] method:

[0062] Five adult chinchillas (body weight 534-689 g) were intraperitoneally injected with carboplatin at a dose of 100 mg per kg body weight, and were killed 48 hours after administration. The other 3 adult chinchillas served as normal controls.

[0063] After the anesthetized animal was decapitated, the auditory bulla was quickly removed. Fix with fixative in a 4°C refrigerator for 1 hour. Separate the basement membrane in PBS solution. Cut the basement membrane into several fragments for different enzymatic cytochemical incubation reactions.

[0064] result:

[0065] Such as figure 2 shown.

[0066] The results showed that ATPase activated by calcium ions was less in the inner and outer hair cells of normal chinchillas, but significantly increased in the inner and outer hair cells of chinchillas with carboplatin ototoxicity.

Embodiment 2

[0068] Vestibular-induced nystagmus duration monitoring

[0069] method:

[0070] Twenty-four adult chinchillas weighing 475-641 grams were equally divided into three groups, with 8 rats in each group. The first group is the normal control group; the second group is the early lesion group; the third group is the late lesion group.

[0071] In the disease group, carboplatin (100 mg / kg) was injected subcutaneously after intraperitoneal injection of methionine (50 mg / kg), or both methionine (50 mg / kg) and carboplatin (100 mg / kg) were injected subcutaneously.

[0072] 4 rats were sacrificed 24 hours and 72 hours after carboplatin injection, this group was the early lesion group; 8 rats were sacrificed 30 days after carboplatin injection, the late stage lesion group.

[0073] The induced nystagmus was detected before the application of methionine and before the sacrifice, and the vestibular terminal apparatus was examined by smear.

[0074] result:

[0075] Such as figure 1 sh...

Embodiment 3

[0079] Symptoms improved after using methionine

[0080] Comparison of cisplatin and cisplatin+methionine for 48-hour cultures of the cochlea and vestibule of three-day postpartum mice.

[0081] method:

[0082] Under aseptic conditions, the cochlea and vestibule were dissected from the inner ear of three-day postpartum mice. The dissected cochlea and vestibule were cultured in culture medium containing 2mM Cisplatin. After 48 hours, they were stained and spread for observation.

[0083] Under sterile conditions, the cochlea and vestibule were dissected from the inner ear of three-day postpartum mice, and the dissected cochlea and vestibule were cultured in a culture medium containing 2mM Cisplatin and methionine, stained and spread for observation after 48 hours.

[0084] result:

[0085] Such as image 3 shown.

[0086] The results showed that the single use of Cisplatin and the combined use of Cisplatin and methionine had no effect on the mouse cochlea;

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Abstract

The invention discloses an application of methionine in preparing the composition for curing or preventing internal acoustic meatus vestibular hair cell diseases. The invention also discloses a use method of the methionine used as therapeutic agent or prophylactic for curing the internal acoustic meatus vestibular hair cell diseases.

Description

technical field [0001] The present invention relates to the field of medicine. It specifically relates to the use of methionine in the preparation of a composition for treating or preventing drug-induced damage to inner ear vestibular hair cells. Background technique [0002] Many diseases involving the inner ear are often accompanied by damage to the vestibular system while causing deafness. Damage to the vestibular system often leads directly to loss of mobility. Symptoms of the vestibular system are often the first reason why patients seek treatment in the ENT department. Therefore, vestibular hair cell injury is a disease that seriously affects human health. [0003] Many anti-tumor chemical preparations, as well as various neurotoxins and noises have certain toxic effects on the cochlea and vestibule. Platinum is an active ingredient in a commonly used and effective clinical chemotherapy agent. Platinum is widely used in anti-tumor platinum preparations. However, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/198A61K9/00A61K9/08A61K9/10A61K9/107A61K9/16A61K9/14A61K9/20A61K9/48A61P27/16
Inventor 曲雁史春梦
Owner 曲雁
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