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Cold adapted endo beta-xylanase gene XynA and use

A technology of xylanase and gene, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of sampling and cultivation difficulties, and less research on polar sea ice microorganisms

Inactive Publication Date: 2011-01-05
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the difficulties of sampling and culturing, there are relatively few studies on polar sea ice microorganisms.

Method used

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  • Cold adapted endo beta-xylanase gene XynA and use
  • Cold adapted endo beta-xylanase gene XynA and use
  • Cold adapted endo beta-xylanase gene XynA and use

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Embodiment 2

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Abstract

The invention relates to a gene XynA of cold adapted endo-beta-xylanase and an application thereof. The genome DNA fragment sequence is shown as SEQ ID N o. 1. The cold adapted beta-xylanase gene XynA is applicable for preparing beta-xylanase XynA. The obtained beta-xylanase XynA is used as neutral cold adapted hydrolase for hydrolyzing oat xylan, beech xylan and birch xylan to produce xylobiose and xylotriose. Optimum enzyme activity temperature is 30 DEG C and optimum pH is 7.0.

Description

A kind of cold-adapted endo-beta-xylanase gene XynA and its application technical field The invention relates to a gene of a novel cold-adaptive endo-xylanase, which belongs to the field of biotechnology. Background technique β-xylanase is a glycoside hydrolase secreted by bacteria and fungi that can degrade xylan. Most of them belong to the 10th and 11th families. In recent years, a few β-xylanases have been found to belong to the 8th family. . The properties of the β-xylanases of the 10th family from different sources are not much different; however, the enzymes of the 11th family have strong substrate specificity, low molecular weight, and the properties of the enzymes of different branches are quite different. The β-xylanases of family 10 can hydrolyze glycosidic bonds adjacent to branch points and non-reducing end glycosidic bonds. For β-xylanases of family 10, two consecutive non-substituted xylose residues between two branch points are sufficient for their action,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/56C12N9/24C12P19/12C12P19/00C12R1/01
Inventor 张玉忠陈波郭兵陈秀兰俞勇周百成
Owner SHANDONG UNIV