Simple method for detecting concentration of oxalic acid
A concentration, oxalic acid technology, applied in the measurement of color/spectral properties, analysis by chemical reaction of materials, etc., can solve the problems of large sample volume, low sensitivity, long reaction time, etc., to reduce test errors and achieve good stability. , the effect of easy promotion
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Embodiment 1
[0023] Embodiment 1 measures the standard sample of known oxalic acid concentration
[0024] (1) React 100 microliters of the sample with excess oxalate oxidase at 40° C., pH 3.5, and 20 mM sodium succinate buffer for 10 minutes;
[0025] (2) adding the hydrogen peroxide and the metal complex formed in step (1) to pH 6.0, 50 mM sodium succinate buffer and reacting at 50° C. for 40 minutes;
[0026] (3) the mixture that measurement step (2) obtains is the absorbance when the ultraviolet absorption wavelength is 450nm; Then with the standard curve of hydrogen peroxide when the wavelength is 450nm, the standard curve contrast of the absorbance can obtain the concentration of oxalic acid in the sample.
Embodiment 2
[0027] Example 2 Determination of oxalic acid concentration in normal human urine sample
[0028] (1) Urine sample pretreatment: first acidify the urine sample with concentrated hydrochloric acid, adjust the pH of the sample to 3.5 with succinic acid, dilute it 3 times, treat the sample with activated carbon, filter, and the filtrate is the pretreated urine sample ;
[0029] (2) Take 500 microliters of the pretreated urine sample and add oxalate oxidase to 30°C, pH is 3.5, and react in 20mM sodium succinate buffer for 10 minutes;
[0030] (3) The hydrogen peroxide generated by the manganese metal complex and step (2) was reacted at 50° C. for 40 minutes at a pH of 6.0, and 2 ml of 50 mM sodium succinate buffer;
[0031] (4) measure the absorbance of step (3) reaction product when ultraviolet absorption wavelength 450nm, compare with the standard curve of absorbance when the standard sample of hydrogen peroxide is 450nm at wavelength, obtain the concentration of oxalic acid in...
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