Method for selectively converting and shunting biological products

A biological product, a selective technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of glucose consumption and inability to use arabinose, and achieve the effect of reducing production costs and improving resource utilization.

Active Publication Date: 2009-08-05
唐传生物科技(厦门)有限公司
View PDF9 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technique allows us to make highly pure sugars like mannitols while minimizing waste during manufacturing processes that use expensive starting compounds such as glucoses. It involves converting them chemically into other molecules called hexamers instead of formaldehydes, resulting in improved yields and lower costs associated with these methods. Additionally, this approach enables efficient recycle of resources without generating excesses of harmful substances. Overall, this technical effect provides an effective solution for making both industrially relevant sources of valuable biochemistry (xymbiose) and industrial applications related thereto.

Problems solved by technology

Technologies discussed include separating yarn cell wall components such as lignocytosilage without decompositing ribonucleotides during acid treatment; enzymatic conversion of α-1 galactobiose to L-2HBGa-35-5'-xylidenide hydrolase and recirculative coolant precipitation methods used alone or combined with another step involving filtrations like centrifuge sedimentation columns. These techniques help separate various ingredients while reducing costs associated with their isolation and reuse.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for selectively converting and shunting biological products
  • Method for selectively converting and shunting biological products
  • Method for selectively converting and shunting biological products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] 40kg corn husks, add 160L of water, boil. Filter off the liquid part, wash the residue once with water, add 80 L of 2% (w / w) sulfuric acid solution to the dried residue, and hydrolyze in a hydrolysis reactor at 125°C for 2 hours. Remove the residue by filtration, adjust the filtrate to pH 3 with calcium carbonate, remove the precipitate by filtration, add 1 kg of activated carbon to the filtrate, remove the activated carbon after adsorption treatment for 30 minutes, and then pass through the cation and anion exchange chromatography columns in turn to obtain a colorless and transparent syrup , and concentrated to the required concentration under reduced pressure. The ratio of glucose, xylose, and arabinose content in this syrup is approximately 1:2:1 (see image 3 ).

Embodiment 2

[0069]Add 280L of 2.4% (w / w) sulfuric acid solution to 40Kg of dried bagasse, and hydrolyze at 125°C for 2 hours. All the other follow-up processing steps are the same as in Example 1. The sugar syrup prepared from bagasse hemicellulose hydrolyzate has a content ratio of glucose, xylose and arabinose of about 1:10:1.

Embodiment 3

[0071] The corn bran hemicellulose hydrolyzate obtained in Example 1 was taken, and the hot water extract from 100 g of rice bran was added to each liter of fermentation broth to adjust the total sugar to 250 g / L, which contained about 120 g / L of xylose and arabinose 80g / L, sterilized at 110°C for 10 minutes. A 5L fermenter (Biotech-5BG; Shanghai Baoxin Bioengineering.Equipment, Shanghai, China) with a capacity of 3L was inoculated with fresh Candida tropicalis CCTCC M 205067 (Candida tropicalis CCTCC M 205067), with an inoculation volume of 5% (v / v), Ventilation volume 1vvm, stirring speed 300rpm, culture at 33°C for about 30h, xylose is converted into xylitol, and arabinose does not participate in the reaction ( Figure 4 ). After the fermentation was stopped, the cell pellet was collected by centrifugation, and the pelleted cells were adjusted into a slurry with fresh medium, and then injected back into the fermenter. The medium was topped up to the fill volume of the fir...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to method for selectively transforming, shunting and preparing various biological products from the same bio-matrixes, in particular relates to a method for directly producing xylitol and arabinose by hemicellulose hydrolysate. The method comprises the following steps: raw materials rich in arabinoxylans are hydrolyzed by acid or enzyme to obtain hydrolysate rich in the arabinose and xylose; and the hydrolysate is inoculated into yeast strains which can ferment the xylose and produce the xylitol and can not utilize the arabinose after detoxification treatment. When the xylose concentration in fermentation liquid is lowered to a certain extend, the fermentation is stopped. Ion-exchange desalinization, discoloration, proper concentration and chromatographic resolution are carried out on the fermentation liquid so as to respectively obtain xylose solution and multi-sugar solution taking the arabinose as main ingredient, from which crystal xylitol and crystal arabinose are respectively obtained by treatment. The method combines the manufacturing processes of the xylitol and the arabinose, thereby reducing the production cost and increasing the use ratio of resources.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner 唐传生物科技(厦门)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products