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Filifolium sibiricum effective component extract as well as preparation method and use thereof

A technology of effective parts and extracts, which is applied in the field of extracts of effective parts of Chinese herbal medicines, can solve the problems of undeveloped extraction technology and achieve the effect of broadening the scope of application

Active Publication Date: 2009-08-12
匡海学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction process of the total flavonoids of clematis is not mature yet

Method used

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  • Filifolium sibiricum effective component extract as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 6,000 g of clematis raw material, first extract 3 times with 50% ethanol which is 20 times the weight of the raw material, each extraction time is 1.5 hours, combine the above extracts, recover ethanol under reduced pressure to less than or equal to 180,000 ml, and obtain medicinal liquid 1;

[0028] Stir the medicinal solution 1 and let it stand for 1 to 2 days. It will naturally settle down to the interface and stop falling, then take the supernatant;

[0029] Continue to recover the solvent from the supernatant until there is no alcohol smell, add water to make the volume to 6000ml, and obtain the medicinal solution 2;

[0030] Load the medicinal solution 2 to D101 type macroporous adsorption resin column chromatography D101 (resin weight 80g, column volume 100ml, column diameter:column height=1:2) for adsorption, the loading flow rate is 1.2 times the column volume per hour, and the upper After the sample is finished, rinse the column with distilled water until...

Embodiment 2

[0033] Take 2500g of raw material of chrysanthemum root and rhizome coarse powder, extract 3 times with 50% ethanol of 20 times the weight of the raw material, each extraction time is 1.5 hours, combine the above extracts, recover ethanol under reduced pressure to less than or equal to 75000ml, and obtain the medicinal solution 1;

[0034] Stir the medicinal solution 1 and let it stand for 1 to 2 days. It will naturally settle down to the interface and stop falling, then take the supernatant;

[0035] Add water to the supernatant to make up to 2500ml to obtain liquid medicine 2;

[0036] Put medicinal solution 2 on AB-8 type macroporous adsorption resin column chromatography (resin weight 80g, column volume 100ml, column diameter:column height=1:2), flow rate is 1.3 times of column volume per hour, use distilled water after sample loading Rinse the column until the water eluate is nearly colorless, discard the water eluate, and elute with 6 times the column volume containing ...

Embodiment 3

[0039]Take 1000g of raw material of chrysanthemum root and rhizome coarse powder, first extract 3 times with 95% ethanol of 20 times the weight of the raw material, each extraction time is 1.5 hours, combine the above extracts, and recover the ethanol under reduced pressure to less than or equal to 30000ml , to obtain liquid medicine 1;

[0040] Stir the medicinal solution 1 and let it stand for 1 to 2 days. It will naturally settle down to the interface and stop falling, then take the supernatant;

[0041] Continue to recover the solvent from the supernatant until there is no alcohol smell, add water to make the volume to 1000ml, and obtain the medicinal solution 2;

[0042] H103 type macroporous adsorption resin column chromatography H103 (resin weight 80g, column volume 100ml, column diameter:column height=1:2) on medicinal liquid 2, flow rate is 1.1 times of column volume per hour, rinse with distilled water after loading the sample Column until the water eluate is nearly...

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Abstract

The invention discloses a filifolium sibiricum effective part extract, a preparation method and an application thereof, belonging to the field of Chinese herbal medicine effective part extracts. The preparation method comprises the following steps: a. using water or ethanol for reflux extraction of filifolium sibiricum for 2-4 times, filtering and merging filtrate; b. recycling solvent from the filtrate till the volume of filtrate is equal to 3-6 times of medicinal materials and obtaining liquid medicine 1; c. adding 10-50 percent ethanol into the liquid medicine 1 for suspension and then standing; d. taking supernatant liquid, to recycle the solvent and obtaining the liquid medicine 2; e. sampling the liquid medicine 2 to the chromatogram of a macroporous resin column for absorption, washing by water till nearly colorless, using 20-60 percent ethanol for elution, merging eluate and concentrating for preparation. The filifolium sibiricum effective part extract contains one effective part group and can effectively treat bacterial infectious diseases. The preparation process of the filifolium sibiricum effective part extract can be applicable to the industrial production, thereby providing sufficient raw materials for the follow-up research of total flavonoids of filifolium sibiricum and broadening the application range of the total flavonoids of filifolium sibiricum.

Description

technical field [0001] The present invention relates to extracts of effective parts of Chinese herbal medicines, in particular to extracts of effective parts of clematis chinensis and a preparation method thereof. The present invention also relates to the use of extracts of effective parts of clematis chinensis in treating bacterial and drug-resistant bacterial infectious diseases, which belongs to Chinese herbal medicine Active parts extract field. Background technique [0002] Filifolium sibiricum (L.) Kitam., also known as Artemisia sibiricum (L.) Kitam.], is a plant of the genus Filifolium sibiricum in the Asteraceae family. It is a natural medicine that is not included in the Pharmacopoeia. The medicine is a heat-clearing and detoxifying medicine, and the whole plant can be used medicinally. Its nature and flavor are bitter and cold, and have the functions of clearing away heat and detoxification, antibacterial and anti-inflammatory, calming the nerves, calming convul...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P31/04A61K125/00
Inventor 匡海学王秋红吕邵娃杨炳友
Owner 匡海学
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