Hair stem cracking liquid

Abstract

The invention discloses hair cracking solution. The cracking solution comprises the following proportions of components: 10mmol / L of Tris-Cl (pH is 8.0), 100mmol / L of NaCl, and 1mmol / L of CaCl2, 20-200mmol / L of DTT, 0.5%-5.0% of SDS (mass concentration), 0.5%-4% of Triton X-100 by volume and 0.2-2mg / ml of protease K, wherein, a solvent is sterile double-distilled water. Compared with the existing hair digestive juice, the cracking solution has the advantages of largely shortened digestion time and greatly improved digestive efficiency, and the cracking solution provides a new way for forensic identification and clinical diagnosis.

Description

technical field [0001] The invention relates to biochemical reagents, in particular to a hair lysate. Background technique [0002] Hair is one of the most common crime scene evidence during crime scene evidence collection. In addition, because the hair shaft has a special molecular structure that makes it non-perishable, it is easier to collect than other crime scene evidence such as blood, saliva, fingerprints, footprints, muscles, etc., especially in crime scenes that have been committed for a long time . At present, in the process of forensic identification, the genetic information of the criminal suspect is obtained from the evidence at the scene, and the molecular comparison is used to determine whether the criminal suspect participated in the crime. In the process of extracting genetic information from the hair shaft as a sample, workers often find that the hair shaft is not easy to digest. The current fastest digestion method still takes 3 hours or even longer. Th...

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of long digestion time and low efficiency of the existing hair, and provide a lysate that can quickly dissolve and digest the hair shaft without destroying the structure of the genetic molecules of the hair shaft

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