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Recombinant adenovirus carrying human lactoferrin, preparation and uses thereof

A technology of human lactoferrin and recombinant adenovirus, applied in the field of cell biology and genetic engineering, can solve undiscovered problems and achieve the effects of inhibiting growth, avoiding weight loss, and protecting immune organs from atrophy

Inactive Publication Date: 2009-08-19
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After searching, no studies have been found on the use of adenovirus as a carrier to mediate human lactoferrin gene drug via peritumoral injection to treat cervical cancer and regulate the body's immunity

Method used

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  • Recombinant adenovirus carrying human lactoferrin, preparation and uses thereof
  • Recombinant adenovirus carrying human lactoferrin, preparation and uses thereof
  • Recombinant adenovirus carrying human lactoferrin, preparation and uses thereof

Examples

Experimental program
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Effect test

experiment example

[0042] Efficacy experiment example: growth inhibition experiment and immune index detection experiment on transplanted U14 cervical cancer solid tumor in mice

[0043] The experiment conducted pharmacological research on the drug of the present invention to observe its influence on transplanted cervical cancer solid tumors in mice and its influence on immune function.

[0044] 1. Material

[0045] 1.1 Animals and cell lines

[0046] Kunming mice (certificate number: SCXK (Beijing) 2004-0001), weighing 18-22 g, were purchased from the Animal Research Institute of Union Hospital. Drink tap water freely, clean animal breeding room, suitable temperature and humidity, and light 8-10h / d. The mouse cervical cancer U14 cell line was quoted from the Institute of Materia Medica, Chinese Academy of Medical Sciences, and was passaged in the abdominal cavity of mice.

[0047] 1.2 Reagents

[0048] Recombinant human lactoferrin adenovirus; recombinant green fluorescent protein adenovirus; anti-...

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PUM

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Abstract

The invention discloses a recombinant adenovirus carrying human lactoferrin; the adenovirus carries lactoferrin hLTF genes and green fluorescence protein GFP genes. The preparation method and applications in medical field of the adenovirus, in particular to the application thereof in growth inhibition of cervical carcinoma solid tumors are also disclosed. The adenovirus can effectively express human lactoferrin around tumor cells, show stable inhibition effects on the growth of the cervical carcinoma solid tumors, have obvious protection effect on the immune organs of tumor organism, obviously increase the ratio of peripheral blood monocyte lymphocyte CD4 to CD8 T lymphocyte, reverse the drifting condition of Th2 of Th1 / Th2 of the tumor organism peripheral blood, and have no obvious effect on the weight growth of mice. The test shows that the adenovirus has obvious tumor resistant effects and enhanced organism immunity and no toxic side effects.

Description

Technical field: [0001] The invention belongs to the technical field of cell biology and genetic engineering, and relates to a medicine for treating cervical cancer solid tumors and a medicine method. The medicine is obtained by operating means. Background technique: [0002] Cervical cancer is a widespread malignant tumor in gynecology. Due to the wide application of vaginal cytology, its incidence has dropped significantly all over the world, but it still ranks first in gynecological malignancies in developing countries. my country’s prevention and treatment of cervical cancer has also achieved great results. However, with the increase in human papillomavirus (HPV) infection rates and changes in social life in some areas, the incidence of cervical cancer has a rising trend, especially in the past 20 years. In recent years, the incidence has obviously tended to be younger, and the proportion of cervical adenocarcinoma has increased. The ratio of squamous cell carcinoma to adenoc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01A61K48/00A61P35/00C12R1/93
Inventor 李青旺李健韩增胜吴国芳胡建宏
Owner NORTHWEST A & F UNIV