Microbial synthesis of D-1,2,4-butanetriol

Abstract

Improved enzyme systems, recombinant cells, and processes employing the same to produce biosynthetic D-1,2,4-butanetriol; D-1,2,4-butanetriol prepared thereby and derivatives thereof; D-1,2,4-butanetriol trinitrate prepared therefrom; and enzymes and genes useful in the enzyme systems and recombinant cells.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Application 60 / 831964, filed July 19,2006. The disclosure of the above application is incorporated herein by reference. [0003] funding [0004] This invention was made with government support under contract N00014-00-1-0825 from the Office of Naval Research with support from the National Science Foundation. The US Government may have certain rights in this invention. technical field [0005] The present disclosure relates to methods and materials for the biosynthesis of 1,2,4-butanetriol and methods and materials for producing 1,2,4-butanetriol trinitrate therefrom, as well as the 1,2 , Biosynthetic methods and materials for compounds that are by-products of the 4-butanetriol biosynthetic system. Background technique [0006] The statements in this section merely provide background information related to the present disclosure and may not constitute prior art....

AI Technical Summary

Problems solved by technology

[0012] A major challenge for such further refinement of the D-1,2,4-butanetriol biosynthetic system lies in the lack of information on catalytic artificial biosynthetic pathways ( figure 1 b) Genetic information of D-xylose dehydrogenase in the first step and the presence of the above-mentioned unexplained catabolic background in the microbial host cell

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