Industrial preparation method for triterpenoid saponin monomer
A technology of total saponins and chlorella saponins, applied in directions such as non-central analgesics, antipyretics, drug combinations, etc., can solve the problems of large dosage of toxic solvents, time-consuming, complicated steps and the like, and achieves low dosage of toxic solvents, Product safety assurance, the effect of simplifying the preparation steps
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Embodiment 1
[0026] Get 10kg of Clematis medicinal material, its source of medicinal material is the dried root and rhizome of Clematis chinensis Osbeck of Ranunculaceae clematis plant, reflux extraction with 5 times the amount of 10% ethanol for 4 times, each time for 1 hour, the extract reduces Concentrate under pressure until there is no alcohol smell, centrifuge at 12,000 rpm at high speed, absorb the supernatant on a HPD-100 macroporous resin column, then wash with water, 20% ethanol, 30%-50%, 70%-90% ethanol in sequence solution until the effluent is colorless, collect 40-50% ethanol eluate, and evaporate to dryness under reduced pressure to obtain the total saponins of Clematis clematis, which are then passed through the HPD-100 column, followed by water, 20%, 30-50%, 90% ethanol solution was eluted until the effluent was colorless, the 30-50% ethanol eluate was collected, evaporated to dryness under reduced pressure, and the extract was further heated and refluxed with 20 times more...
Embodiment 2
[0039]Take 10 kg of Clematis medicinal material, the source of which is the dry root and rhizome of Clematis hexapetala Pall. Decoct and extract 3 times with 10 times the amount of water, 2 hours each time, after the extract is concentrated, high-speed centrifugation (speed 12000 rpm or more) or filtration, after the filtrate is concentrated, it is absorbed by D-101 macroporous resin, and then water , 20%, 30-50%, 70-95% ethanol solution until the effluent is colorless, collect 30-50% ethanol eluate, and evaporate to dryness under reduced pressure to obtain total saponins of Clematis, total saponins of Clematis Heat and reflux extraction with 20 times the amount of ethanol solution above 80% for 4 times, each time for 2 hours, combine the extracts, concentrate under reduced pressure to 2 / 10 of the total volume, place at 5 ° C for 10 hours, and centrifuge at a high speed (12000 rpm More than / minute) or filter, the precipitation is washed 2 times with more than 80% ethanol of ...
Embodiment 3
[0041] Take 10 kg of Clematis medicinal material, the source of which is the dry root and rhizome of Clematis manshurica Rupr. Cold-soak with 8 times the amount of 20% ethanol solution twice, each time for 24 hours, the extract is centrifuged at a high speed (above 8000 rpm), filtered, and the supernatant is concentrated to dryness under reduced pressure to obtain an extract. Dissolve the extract in water and put it on the D-101 macroporous resin column. Water, 10%, 20-50% and 95% ethanol were eluted respectively, the 30-50% ethanol eluate was collected, and the solvent was recovered under reduced pressure to obtain total saponins of Clematis clematis. The total saponins of Clematis clematis are passed through a D-101 macroporous resin column, and then eluted with water, 20%, 30-50%, and 90% ethanol in sequence until the effluent is colorless, and the 30-50% ethanol eluate is collected and depressurized. Evaporate to dryness, the extract is further heated and refluxed with mo...
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