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Purification method for nicotine conversion rate characteristic of tobacco variety

A technology of nicotine conversion rate and purification method, which is applied in the field of purification of tobacco nicotine conversion rate traits, can solve the problems of not effectively controlling and reducing the nicotine conversion rate of tobacco leaves and reducing nicotine content, so as to reduce the nicotine conversion rate , reduce the proportion, facilitate the effect of promotion

Inactive Publication Date: 2009-09-23
TOBACCO RES INST OF HUBEI PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no technology to effectively control and reduce the nicotine conversion rate and nornicotine content of tobacco leaves in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: get burley tobacco as purification object, and this purification method is carried out by following steps: 1, identification period, tobacco strain group tree stage, is after transplanting 30 days, plant height about 34cm, 14 leaves of every plant, All the tobacco plants in the plantation garden were taken from the bottom to the top and 2 leaves were grown at the ninth leaf position, and all the sampled plants were numbered and listed; for sample processing, the picked tobacco leaves were sprayed with 1% ethephon aqueous solution and then dried in the air for 5 days. After the tobacco leaf turns yellow completely, remove the main vein and dry it in time, crush it to make a powder sample for later use; measure, weigh 100 mg of each sample, extract nicotine and nornicotine with chloroform, and use gas chromatography for automatic analysis The content of nicotine and nornicotine was measured by a meter; the specific operation and parameter setting were carried...

Embodiment 2

[0031] Embodiment 2: get Maryland tobacco as purification object, and this purification method operation step is: 1, identification period, the period of tobacco plant cluster is 28 days after transplanting, and tobacco plant height is about 33cm, and every plant blade 13; For all tobacco plants, take 2 fixed leaves at the 8th leaf position from the lower cloth, and all the sampled plants are numbered and listed; for sample treatment, the tobacco leaves are picked and sprayed with 1% ethylene solution, then moisturized and dried for 4 days, and the tobacco leaves turn yellow completely. , remove the main vein and dry it in time, and make a powder sample for later use; other operating steps, required equipment, conditions, etc. are all the same as in Example 1.

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PUM

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Abstract

The invention relates to a purification method for the nicotine conversion rate characteristic of a tobacco variety. The method comprises the following operational steps: identifying field tobacco conversion plants, determining a rosette stage as an identification stage which is 25 to 30 days after the transplantation of the tobacco plants, setting the height of each plant about 33 cm, and having about 13 leaves for each plant; carrying out sampling, sample processing, nicotine and nornicotine extraction and determination, the calculation of the nicotine conversion rate and the removal of converted plants so as to eliminate the tobacco plants with the nicotine conversion rate more than 5 percent; and marking the seed: marking the letters of 'LC' onto the tobacco variety after the nicotine conversion rate purification. The method has simple operation and convenient popularization. The method can be used for identifying and removing nicotine converted plants, reducing the proportion of the converted plants and the nicotine conversion rate, and improving the safety of the tobacco leaves. The method is suitable for the low nicotine conversion rate characteristic purification and variety selection aiming at reducing nitrosamine.

Description

technical field [0001] The invention relates to a method for purifying the properties of the nicotine conversion rate of tobacco, in particular to a method for purifying the properties of the nicotine conversion rate of tobacco varieties. Background technique [0002] Tobacco alkaloids mainly include nicotine, nornicotine, anatabine and anatabine. Common tobacco belongs to various types of nicotine, mainly nicotine, whose content accounts for more than 92% of the total alkaloids, and other alkaloids only account for a small part. The level of nicotine content is an important factor to determine the sensory quality of tobacco leaves. Generally, it is required that the content of nicotine should be appropriate to achieve the purpose of moderate physiological strength, mellow taste and low irritation. Nornicotine is also known as demethylated nicotine. Under normal circumstances, the content of reduced nicotine does not exceed 3.5% of the total biological content. It is mainly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04G01N33/00
Inventor 李进平李宗平
Owner TOBACCO RES INST OF HUBEI PROVINCE