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Pretreatment method for dehydroepiandrosterone in health foods

A technology for dehydroepiandrosterone and health food, applied in the field of food analysis, can solve the problems of inability to perform instant detection, consumption of large organic solvents, complicated operation, etc., to achieve a stable baseline, reduce sample loss, and reduce the effect of interference

Inactive Publication Date: 2011-11-16
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Liquid-liquid extraction, this method is cumbersome, time-consuming, and consumes a large amount of organic solvents, resulting in high cost and environmental pollution, and often has emulsification. Using this purification method is time-consuming and laborious, and it cannot be detected immediately. It has been gradually adopted by some other The extraction method is replaced by

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  • Pretreatment method for dehydroepiandrosterone in health foods
  • Pretreatment method for dehydroepiandrosterone in health foods
  • Pretreatment method for dehydroepiandrosterone in health foods

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A pretreatment method for dehydroepiandrosterone samples in health food, comprising the steps of:

[0028] (1) Extraction of dehydroepiandrosterone samples in health food: Take 1g of the slimming tea sample crushed through an 80-mesh sieve into a stoppered container, add 10mL of methanol, place it on a KES-1000 ultrasonic cleaning machine, and ultrasonicate for 20min at 3000r Centrifuge at 1 / min for 3 min, take the supernatant, extract repeatedly three times, combine the supernatants to obtain a sample extract containing dehydroepiandrosterone, and spin evaporate to dryness at 40°C to obtain an evaporated sample for later use.

[0029] (2) Purification of dehydroepiandrosterone solid-phase extraction in health food: use 5 mL of ddH with 20% methanol by volume for the evaporated sample 2 O was dissolved to obtain the sample extract after reconstitution; carry out solid phase extraction through the C18 solid phase extraction column according to the following procedure, th...

Embodiment 2

[0042] A pretreatment method for dehydroepiandrosterone samples in health food, comprising the steps of:

[0043] (1) Extraction of dehydroepiandrosterone samples in health food: Take 1g of the slimming tea sample crushed through an 80-mesh sieve into a stoppered container, add 5mL of methanol, place it on a KES-1000 ultrasonic cleaning machine, and ultrasonicate for 10min at 3000r Centrifuge at 1 / min for 3 min, take the supernatant, extract repeatedly three times, combine the supernatants to obtain a sample extract containing dehydroepiandrosterone, and spin evaporate to dryness at 40°C to obtain an evaporated sample for later use.

[0044] (2) Purification of dehydroepiandrosterone solid-phase extraction in health food: use 5 mL of ddH with 30% methanol by volume to evaporate the dried sample 2 O was dissolved to obtain the sample extract after reconstitution, and carried out solid-phase extraction through the C18 solid-phase extraction column according to the following proc...

Embodiment 3

[0057] A pretreatment method for dehydroepiandrosterone samples in health food, comprising the steps of:

[0058] (1) Extraction of dehydroepiandrosterone samples in health food: Take 0.5g of the slimming tea sample crushed through an 80-mesh sieve into a stoppered container, add 8mL of methanol, place it on a KES-1000 ultrasonic cleaner, and ultrasonicate for 15min. Filter, take the supernatant, filter repeatedly three times, combine the supernatants to obtain a sample extract containing dehydroepiandrosterone, and spin evaporate to dryness at 40°C to obtain an evaporated sample for later use.

[0059] (2) Purification of dehydroepiandrosterone solid-phase extraction in health food: use 5 mL of ddH with 25% methanol by volume for the evaporated sample 2 O was dissolved to obtain the sample extract after reconstitution, and carried out solid-phase extraction through the C18 solid-phase extraction column according to the following procedure, the specific method is:

[0060] (a...

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Abstract

The invention provides a pretreatment method for dehydroepiandrosterone in health foods, belonging to the filed of food analysis. The method comprises the following steps: weighting a certain quality of health food samples; adding a certain volume of methanol; extracting the dehydroepiandrosterone in the samples under the ultrasound for 10 min to 20 min; and adopting a C18 solid-phase column extractor to treat the extracted samples so as to achieve the aim of purification. The treated samples are suitable for sample treatment, such as the detection of dehydroepiandrosterone in the health foods by a high-efficiency liquid-phase method, an enzyme-linked immunoadsorption method, and the like. The invention greatly reduces the interference of a sample substrate and remarkably improves the sensitivity of the detection.

Description

technical field [0001] The invention belongs to the field of food analysis, in particular to a pretreatment method for solid-phase extraction of dehydroepiandrosterone in health food and its application. Background technique [0002] DHEA is a kind of human adrenaline, which has certain effects on the prevention and treatment of cardiovascular diseases and enhancement of immunity. It is not allowed to be added to health food at present. [0003] The national standard of the People's Republic of China stipulates the determination method of dehydroepiandrosterone in health food, which mainly adopts a simple extraction method for determination, but the composition of health food is complex, and many interfering substances will be extracted at the same time when methanol is used to extract, thus Seriously affect the accuracy and sensitivity of detection. Therefore, the samples need to be purified. [0004] Liquid-liquid extraction, protein precipitation, and solid-phase extrac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N33/02A23L5/20
Inventor 雷红涛孙远明石敏沈玉栋张挺杨金易王弘徐晓艳
Owner SOUTH CHINA AGRI UNIV