Method for preparing germs polyclonal antibody of sugarcane ratoon stunting disease

Abstract

The invention relates to the technical field for preventing and curing sugarcane ratoon stunting disease, providing a method for preparing germs polyclonal antibody of sugarcane ratoon stunting disease, comprising the following steps: (1) culturing pathogenic germs by means of isolation to obtain antigen; (2) mixing the antigen with equivalent Freund complete adjuvant, emulsifying, and immunizing rabbit; (3) mixing the antigen with equivalent Freund incomplete adjuvant, emulsifying, and again immunizing rabbit, repeating the immunizing process several times, after measuring of titer meets requirement, collecting blood from the heart, isolating antiserum; and (4) purifying the antiserum to obtain the polyclonal antibody. In the invention, the germs of the sugarcane perennial root dwarfing is isolated from sugarcane juice, thus the polyclonal antibody which is obtained thereby can specifically identify the germs of the sugarcane ratoon stunting disease in the sugarcane juice. The germs polyclonal antibody of the sugarcane ratoon stunting disease has the advantages of high specificity, high purity and high titer, can be preserved for a long time, and has important practical significance in measuring the pathogenic germs to diagnose the sugarcane ratoon stunting disease (RSD) by means of immunology in the scientific research and the production of the sugarcane.

Description

Technical field

[0001] The invention relates to the technical field of polyclonal antibody preparation, in particular to a preparation method of polyclonal antibody against sugarcane ratoon stunting disease bacteria. Background technique

[0002] Sugarcane ratoon stunting disease (RSD) is one of the most important bacterial diseases in sugarcane production. The pathogen Lxx is very small and parasitizes in the xylem ducts of sugarcane. Because the disease has no obvious external and internal symptoms, and it is easy to be confused with the symptoms caused by drought, and it is mainly spread through germ-carrying seed stems and cutting tools, which leads to unintentional transmission, causing the disease to spread, and harming sugarcane production. great. The disease usually causes sugarcane yield to be reduced by 12-37%. Under drought conditions, the yield is reduced by up to 60%, and the ratoon is weakened. Even disease-free healthy seedlings are 100% susceptible after being use...

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