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Oleander oligosaccharide, preparation method thereof and use thereof

A technology of oleander and oligosaccharides, which can be applied to pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., and can solve the problems of low biopharmaceutical efficiency

Inactive Publication Date: 2011-09-07
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problem encountered is that if these macromolecules are administered orally, their bioavailability will be very low

Method used

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  • Oleander oligosaccharide, preparation method thereof and use thereof
  • Oleander oligosaccharide, preparation method thereof and use thereof
  • Oleander oligosaccharide, preparation method thereof and use thereof

Examples

Experimental program
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Embodiment 1

[0028] a. Polysaccharide extraction:

[0029] Dried oleander flowers were degreased with 95% ethanol for one week, and then dried naturally at room temperature. 961 g of dried oleander flowers were extracted 5 times with 20 liters of boiling water, each for 6 hours. Sulfuric acid-phenol detects that there is no obvious reaction, filter, combine the extracts each time, heat and concentrate to 3 liters, add about 500g trichloroacetic acid after cooling to make the final concentration 15%, deproteinize at 4°C, 1mol / Neutralize with L NaOH to a pH value of 7.0, then dialyze against flowing water for 72 hours, concentrate the solution inside the dialysis bag to a volume of 2 liters, add three times the volume of 6 liters of 95% ethanol under stirring, let it stand overnight, pour off the The supernatant was separated by centrifugation, and the obtained precipitate was washed with 2 times the volume of absolute ethanol, separated by centrifugation, and the precipitate was vacuum-dr...

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Abstract

The invention provides two kinds of oligosaccharide obtained from oleander flowers, a preparation method thereof and use thereof. Particularly, the two kinds of oligosaccharide are prepared by firstly extracting polysaccharide from the oleander flowers, and then carrying out hydrolysis, separation and purification of column chromatography. In-vitro experiments show that the two kinds of oligosaccharide have an obvious anti-angiogenesis effect, and are expected to become an anti-tumor medicament.

Description

technical field [0001] The invention relates to sugars and their application in the preparation of medicines, in particular to two oleander oligosaccharides and their preparation methods and applications. Background technique [0002] Sugar is the three basic elements of life, and plays an important role in many life processes. In addition to the mature anticoagulant drug heparin and acarbose for the treatment of diabetes, there are 34 sugar drugs currently in Phase I-III clinical trials. The biggest feature of sugar drugs is low toxicity or non-toxicity, so they have great application prospects. [0003] Polysaccharides derived from oleander flowers have anti-tumor, antioxidant, immune-promoting and neuroprotective effects. However, the problem encountered is that if these macromolecules are administered orally, their bioavailability will be very low. Oligosaccharides have various biological activities and are a relatively new functional product, which are used in health...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 丁侃胡珂刘芹王硕
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI