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mundoserone inhibits angiogenesis in zebrafish embryos

A technology of 1.mundoserone and 2.mundoserone, applied in the application field of disease medicine

Active Publication Date: 2019-12-31
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the relationship between Mundoserone and angiogenesis based on the model animal zebrafish

Method used

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  • mundoserone inhibits angiogenesis in zebrafish embryos
  • mundoserone inhibits angiogenesis in zebrafish embryos
  • mundoserone inhibits angiogenesis in zebrafish embryos

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Experimental program
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Embodiment 1

[0024] 1. Materials and methods

[0025] 1. Zebrafish Husbandry and Breeding

[0026] The fli1a-EGFP transgenic zebrafish expressing green fluorescent protein in vascular endothelial cells was used. Adult zebrafish, larvae, and embryos were all kept in culture water at 28°C and pH 7.0, and the rearing room was routinely set to 14 hours of light and 10 hours of darkness to simulate a day and night cycle. When breeding, 5-6 pairs of zebrafish are mated each time. Generally, each pair of zebrafish can produce 200-300 eggs at a time. Zebrafish breeding equipment and breeding methods are in compliance with the regulations of the International Laboratory Animal Assessment and Accreditation Committee (AAALAC).

[0027] 2. Drug configuration and use

[0028] Mundoserone was purchased from Microsource, the positive control drug PTK787 was purchased from Selleckchem, and the solvent dimethyl sulfoxide (DMSO) was purchased from Sigma. When preparing, Mundoserone and PTK787 were first...

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PUM

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Abstract

The invention relates to the application of Mundoserone to inhibition of zebrafish embryo angiogenesis. Zebrafish is put into Mundoserone medicine bath with different concentration, a medicine with specific anti-angiogenesis effect serves as positive control, a solvent serves as negative control, and the Mundoserone has the effect of inhibiting zebrafish embryo angiogenesis through discovery; furthermore, the inhibition rate is related to the medicine concentration, and on this basis, the influence on the related signal channel in the zebrafish embryo angiogenesis process by the Mundoserone isresearched through Real-time PCR, and the mechanism for inhibiting angiogenesis is defined. Therefore, the Mundoserone can be used for preparing related medicines and used for treating diseases taking angiogenesis as pathological characteristics, such as tumor, diabetic retinopathy, hemangioma, psoriasis, rheumatoid arthritis and menstrual disorder.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the effect of Mundoserone in inhibiting zebrafish embryo angiogenesis and its application in the preparation of medicines for treating diseases characterized by angiogenesis. Background technique [0002] Angiogenesis plays an important role in the progression of malignant tumors. New blood vessels provide nutrients for tumor cells, so that they can continue to proliferate; on the contrary, if the formation of new blood vessels is inhibited, tumor cells may stop proliferating or even appear necrosis if they do not receive enough nutrients. Therefore, inhibiting angiogenesis is a method to treat malignant tumors, and drugs that inhibit angiogenesis may become potential antitumor drugs. [0003] Chinese patent document CN100577180C discloses the application of gambogic acid in the preparation of drugs for inhibiting angiogenesis. The application of formononetin in the prepara...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/353A61P35/00A61P9/14A61P17/06A61P19/02A61P29/00A61P15/00
CPCA61K31/353
Inventor 王长谦陈侃范虞琪
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE