Application of tanshinlactone in preparation of anti-tumor-angiogenesis medicament
An angiogenesis and anti-tumor technology, applied in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc.
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Embodiment 1
[0019] Example 1: Preparation of salvianolide (the salvianolide used in the following examples was prepared by the following method)
[0020] Using Salvia yunnanensis (Labiatae sage) Salvia yunnanensis C. H. Wright) 10 kg of dried roots or rhizomes, crushed into coarse powder, extracted by cold immersion in 25L of acetone, repeated three times, concentrated to obtain crude extract; the extract was made into suspension with water, extracted with ethyl acetate to obtain ethyl acetate Section; this section adopts silica gel column chromatography, petroleum ether-ethyl acetate gradient elution, obtains 5 partial elution sections, wherein 9:1 elution part adopts silica gel chromatography column again, chloroform-acetone gradient elution (using successively 8:1, 5:1, 3:1 elution), the 5:1 elution fraction was eluted with RP-C 18 Column chromatography methanol-water gradient elution (elution with 80:20, 85:15 in sequence), the fraction eluted at 85:15 was separated and purified by...
Embodiment 2
[0027] Example 2: Effect of salvianolide on the proliferation of human liver cancer cell line HepG-2
[0028] The human liver cancer cell line HepG-2 was incubated at 37°C in 5% CO 2 Cultivate for 24 hours, inoculate it into a 96-well plate according to the inoculation amount of 3000 per well, and after culturing for 24 hours under the above conditions, make salvianolide into different concentrations of 0.5, 1.0, 2.5, 5.0, 10.0ug / mL Concentration solution was added to a 96-well plate, at 37°C, 5% CO 2 The culture was continued for 72 hours under certain conditions, the supernatant was carefully removed, 150 μL DMSO was added to each well, shaken for 1 hour, and the absorbance A value was measured at 490 nm on a microplate reader.
[0029] Such as figure 2 The results showed that the absorbance of the MTT experiment decreased significantly when the salvianolide concentration was 5.0ug / mL and 10ug / mL, indicating that the proliferation activity of tumor cells decreased sign...
Embodiment 3
[0033] Embodiment 3: chicken embryo allantoic membrane experiment of salvianolide, concrete operation is as follows:
[0034] 1. Purchase fresh eggs produced within three days, wipe and disinfect the surface of the eggs with 84 disinfectant or bromogeramine, then put the eggs into a constant temperature incubator, set the temperature at 37.8°C, and maintain a certain humidity (60%). Under this condition Incubate for 7 days.
[0035] 2. Opening the window and administering the drug
[0036] 2.1 Preparation of blank control solution, positive control solution and salvianolide solution: (1) Preparation of blank control solution: use blank saline as a control; (2) Preparation of positive control solution: use 5 mg / mL dexamethasone sodium phosphate injection as a positive control ; (3) Preparation of Salvia lactone solution: Accurately weigh 2 mg of Salvia lactone and configure it with absolute ethanol to make a 1 mg / mL mother liquor for later use, use the above mother liquor to dil...
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