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Application of Mundoserone to inhibition of zebrafish embryo angiogenesis

A technology of zebrafish embryo and angiogenesis, applied in the field of biomedicine

Active Publication Date: 2018-06-29
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the relationship between Mundoserone and angiogenesis based on the model animal zebrafish

Method used

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  • Application of Mundoserone to inhibition of zebrafish embryo angiogenesis
  • Application of Mundoserone to inhibition of zebrafish embryo angiogenesis
  • Application of Mundoserone to inhibition of zebrafish embryo angiogenesis

Examples

Experimental program
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Embodiment 1

[0024] 1. Materials and methods

[0025] 1. Zebrafish breeding and reproduction

[0026] Using fli1a-EGFP transgenic zebrafish expressing green fluorescent protein with vascular endothelial cells, adult zebrafish, juveniles and embryos are all reared in 28℃, pH7.0 culture water, and the rearing room is routinely set for 14 hours of light and 10 hours of darkness To simulate the day and night cycle. When breeding, take 5-6 pairs of zebrafish each time for mating, generally each pair of zebrafish can lay 200-300 eggs at a time. Zebrafish breeding equipment and breeding methods are in compliance with the requirements of the International Laboratory Animal Evaluation and Accreditation Committee (AAALAC).

[0027] 2. Drug configuration and use

[0028] Mundoserone was purchased from Microsource, the positive control drug PTK787 was purchased from Selleckchem, and the solvent dimethyl sulfoxide (DMSO) was purchased from Sigma. When configuring, dissolve Mundoserone and PTK787 in DMSO fir...

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Abstract

The invention relates to the application of Mundoserone to inhibition of zebrafish embryo angiogenesis. Zebrafish is put into Mundoserone medicine bath with different concentration, a medicine with specific anti-angiogenesis effect serves as positive control, a solvent serves as negative control, and the Mundoserone has the effect of inhibiting zebrafish embryo angiogenesis through discovery; furthermore, the inhibition rate is related to the medicine concentration, and on this basis, the influence on the related signal channel in the zebrafish embryo angiogenesis process by the Mundoserone isresearched through Real-time PCR, and the mechanism for inhibiting angiogenesis is defined. Therefore, the Mundoserone can be used for preparing related medicines and used for treating diseases taking angiogenesis as pathological characteristics, such as tumor, diabetic retinopathy, hemangioma, psoriasis, rheumatoid arthritis and menstrual disorder.

Description

Technical field [0001] The invention relates to the technical field of biomedicine, in particular to the effect of Mundoserone in inhibiting zebrafish embryo angiogenesis, and its application in preparing medicines for treating diseases characterized by angiogenesis. Background technique [0002] Angiogenesis plays an important role in the progression of malignant tumors. New blood vessels provide nutrients for tumor cells, enabling them to continue to proliferate; on the contrary, if the formation of new blood vessels is inhibited and tumor cells cannot get enough nutrients, they may stop proliferating and even cause necrosis. Therefore, inhibition of angiogenesis is a method for the treatment of malignant tumors, and drugs that inhibit angiogenesis may become potential anti-tumor drugs. [0003] Chinese patent document CN100577180C discloses the application of gambogic acid in the preparation of drugs for inhibiting angiogenesis. The invention provides the application of gambogi...

Claims

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Application Information

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IPC IPC(8): A61K31/353A61P35/00A61P9/14A61P17/06A61P19/02A61P29/00A61P15/00
CPCA61K31/353
Inventor 王长谦陈侃范虞琪
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE