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Preparation method and application of functionalized nano-carbon tube anti-neoplastic drug carrier

A technology of carbon nanotubes and anticancer drugs, which is applied in the field of preparation of functionalized carbon nanotube anticancer drug carriers, can solve the problems that functionalized carbon nanotube anticancer drug carriers are rarely reported, and achieves simple and effective preparation methods obvious effect

Inactive Publication Date: 2010-01-06
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application research of nanomaterials in the field of biomedicine is mostly based on zero-dimensional nanoparticles, but there are few reports on the development and development of functionalized carbon nanotubes as anticancer drug carriers by using their special structural characteristics.

Method used

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  • Preparation method and application of functionalized nano-carbon tube anti-neoplastic drug carrier
  • Preparation method and application of functionalized nano-carbon tube anti-neoplastic drug carrier
  • Preparation method and application of functionalized nano-carbon tube anti-neoplastic drug carrier

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Prepare the functionalized carbon nanotube anticancer drug carrier according to the following steps:

[0050] (1) Mix 15ml of sulfuric acid (98%) and 5ml of nitric acid (63%) to prepare a mixed acid; place 20mg of carbon nanotubes (MWNTs) in the mixed acid, and ultrasonically treat it at 50Hz for 16 hours;

[0051] (2) centrifuging the above mixture; washing the separated solid matter with distilled water to neutrality to obtain short carbon nanotubes;

[0052] (3) Take 10 mg of short carbon nanotubes, mix with 50 mg of triblock copolymer (Pluronic P123) aqueous solution, and ultrasonically treat at 50 Hz for 3 hours;

[0053] (4) The above mixture is filtered with a 200nm microporous membrane, the filtrate is discarded, the obtained substance is centrifuged, and then placed in 10ml of water to obtain a multi-walled carbon nanotube system (P123-MWNTs) uniformly dispersed in the aqueous solution;

[0054] (5) The above-mentioned aqueous phase system is sterilized by mem...

Embodiment 2

[0059] Prepare the functionalized carbon nanotube anticancer drug carrier according to the following steps:

[0060] (1) Mix 30ml of sulfuric acid (98%) and 10ml of nitric acid (63%) to prepare a mixed acid; place 30mg of carbon nanotubes (MWNTs) in the mixed acid, and ultrasonically treat it at 50Hz for 16 hours;

[0061] (2) centrifuging the above mixture; washing the separated solid matter with distilled water to neutrality to obtain short carbon nanotubes;

[0062] (3) Take 15 mg of short carbon nanotubes, mix with 100 mg of triblock copolymer (Pluronic P123) aqueous solution, and ultrasonically treat at 50 Hz for 3 hours;

[0063] (4) The above mixture is filtered with a 200nm microporous membrane, the filtrate is discarded, and the obtained substance is centrifuged, and then placed in 20ml of water to obtain a multi-walled carbon nanotube system (P123-MWNTs) uniformly dispersed in the aqueous solution;

[0064] (5) The above-mentioned aqueous phase system is sterilized ...

Embodiment 3

[0069] Prepare the functionalized carbon nanotube anticancer drug carrier according to the following steps:

[0070] (1) 45ml of sulfuric acid (98%) and 15ml of nitric acid (63%) were mixed to prepare mixed acid; 40mg of carbon nanotubes (MWNTs) were placed in the mixed acid, and 50Hz power ultrasonic treatment was performed for 16 hours;

[0071] (2) centrifuging the above mixture; washing the separated solid matter with distilled water to neutrality to obtain short carbon nanotubes;

[0072] (3) Take 20 mg short carbon nanotubes, mix with 150 mg triblock copolymer (Pluronic P123) aqueous solution, and ultrasonically treat at 50 Hz for 3 hours;

[0073] (4) The above mixture is filtered with a 200nm microporous membrane, the filtrate is discarded, and the obtained substance is centrifuged, and then placed in 75ml of water to obtain a multi-walled carbon nanotube system (P123-MWNTs) uniformly dispersed in the aqueous solution;

[0074] (5) The above-mentioned aqueous phase sy...

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Abstract

The invention relates to a preparation method of functionalized nano-carbon tube antineoplastic drug carrier and an application of functionalized nano-carbon tube camptothecin composite in treating Hela cervical carcinoma cells in vitro, belonging to an antineoplastic drug carrier. The preparation method comprises the following steps: mixing sulphuric acid with nitric acid to prepare mixed acid; putting a nano-carbon tube into the mixed acid for carrying out ultrasonic processing; separating centrifugally; washing the mixture with distilled water to be neutral to obtain a short nano-carbon tube; mixing the short nano-carbon tube with triblock copolymer water solution; using a 200 nm microporous filtering film to filter to obtain multi-wall nano- carbon tube aqueous phase system; adding camptothecin solution into the aqueous phase system for centrifugal separation and filtration to obtain triblock copolymer nano-carbon tube camptothecin; and uniformly scattering the complex into physiological saline to obtain stable camptothecin multi-wall nano- carbon tube complex. The invention has the advantages of obvious tumor cell inhibition in treating Hela cervical carcinoma cells in vitro, broad-spectrum anti-tumor activity and simple preparation method.

Description

technical field [0001] The invention belongs to an anticancer drug carrier, in particular to a preparation method of a functionalized carbon nanotube anticancer drug carrier and the application of a functionalized carbon nanotube camptothecin compound in the treatment of Hela cervical cancer cells in vitro. Background technique [0002] Cancer is a disease that seriously endangers human health in contemporary times. Currently, the three major medical methods for treating cancer: surgery, chemotherapy, and radiotherapy all have obvious adverse effects on the human body. Therefore, how to effectively treat cancer and invent a kind of anticancer drug with good therapeutic effect and no side effects is a major scientific problem of common concern to mankind. [0003] Using natural organisms as raw materials to prepare new drugs with anticancer activity is an important content of anticancer medicine research. With the help of modern extraction and modification technology, effect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K47/04A61K31/4745A61P35/00A61K47/52
Inventor 贾能勤田忠
Owner SHANGHAI NORMAL UNIVERSITY
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