Gene for coding sucrase and application thereof

Abstract

The invention discloses a gene inv2 for coding sucrase, having a nucleotide sequence described by a sequence table SEQ ID NO.1 or the sucrase is coded by a function equal variant having the nucleotide sequence ofSEQ ID NO.2. The sucrase coded by the gene or the function equal variant of the gene has a wide application in degradation of sucrose.

Description

technical field [0001] The present invention relates to a gene encoding sucrase, in particular to a new gene encoding sucrase cloned from environmental metagenome, the protein encoded by the gene can be used for the degradation of sucrose. Background technique [0002] Sucrose is a renewable carbohydrate produced by photosynthesis in plants, with an annual global production of more than 500 million tons (Marquez Savaya Jacques, China-Pakistan Cooperation on Sucrose Ethanol, Business Weekly, 2009, 1:72) . The enzymatic hydrolysis of sucrose is an important biochemical reaction process: sucrose is decomposed by the hydrolysis of sucrose hydrolase-β-fructofuranosidase or sucrase (β-fructofuranosidase, Invertase, EC 3.2. and fructose), monosaccharides are further used to produce important industrial products such as fuel ethanol, so efficient sucrose hydrolase is a key factor for the utilization of sucrose bioenergy (Marris E, Sugar cane and ethanol: drink the best and drive th...

AI Technical Summary

Problems solved by technology

However, the microorganisms that can be cultivated by humans in nature only account for 1% of the microbial species in nature (Rappé MS, Giovannoni SJ. The uncultured microbial majority. Annu. Rev. Microbiol., 2003, 57: 369-94), while the remaining 99% Microorganisms cannot be cultivated under current experimental conditions