Device for detecting three-dimensional growth of nerve cells and electric signals thereof
A growth device and nerve cell technology, applied in measuring devices, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve problems that restrict the development of nerve cell electrophysiology
Inactive Publication Date: 2010-02-03
ZHEJIANG UNIV
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Problems solved by technology
Therefore, to a certain extent, there is a difference between the current scientific research on nerve cell electrophysiology based on MEA technology and the neural network with orderly connections in the actual space in vivo. The current planar culture technology restricts the research on nerve cell electrophysiology. develop
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[0021] figure 1 , figure 2 and image 3 The structure of a first embodiment of the device according to the invention is shown. The device includes a bottom cell growth device 2 , a top cell growth device 1 , a bottom microelectrode array sensor 42 , a top micro electrode array sensor 41 and a connector 3 . Both the bottom cell growth device 2 and the top cell growth device 1 include microchambers arranged in a matrix. The following examples illustrate that when the bottom cell growth device 2 and the top cell growth device 1 are as figure 1 and Figure 7 When nine microchambers are shown, the nine microchambers are arranged in a matrix of three rows and three columns. The microchambers of the bottom cell growth device 2 are up and down relative to the corresponding microchambers of the top cell growth device 1, so that the nerve cells cultured in the microchambers of the top cell growth device 1 and the neurons in the microchambers of the bottom cell growth device 2 Syn...
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The invention discloses a device for detecting three-dimensional growth of nerve cells and electric signals thereof, which is characterized in that more than two layers of cell growing devices are fixedly connected between two layers of microelectrode array sensors, each cell growing device is provided with microchambers which are arranged in a matrix mode, the corresponding microchambers of the cell growing devices at the different layers are opposite up and down, and every two adjacent microchambers on the same line and the same row of each cell growing device are communicated by a microchannel; a large microelectrode is correspondingly arranged in each of the microchambers of the cell growing devices at the bottom layer and the top layer, and a small microelectrode is correspondingly arranged in each microchannel; a communicating vessel is arranged between two layers of cell growing devices and is provided with a micro through hole matrix corresponding to the upper microchamber andthe lower microchamber; a channel is formed among the microchambers which are mutually corresponding up and down and the micro through holes of the communicating vessel; the size of each microchamberonly allows to contain one nerve cell; and the width of each microchannel only allows to contain one nerve synapse. The device ensures that the cultured nerve cells can be sequentially connected in the three-dimensional space, and can detect the electric signals of the nerve cells.
Description
technical field [0001] The invention relates to a device for detecting nerve cell electrical signals, in particular to a device for detecting nerve cell electrical signals using a micro-electrode array sensor. Background technique [0002] The analysis of neural electrical signals is one of the main contents of neuroinformatics research. A more commonly used device is a micro-electrode array sensor (Micro-Electrode Array, MEA). MEA consists of microelectrodes embedded in a substrate material. Microelectrodes are mainly composed of metal materials such as platinum, gold, titanium nitride, and indium tin oxide. Microelectrodes are arrayed on a substrate material (usually glass). The microelectrodes are connected to wires made of gold or transparent indium tin oxide, which carry microelectrode signals to an external amplifier, or externally applied stimuli to the microelectrodes. In the MEA device, the cells or tissues to be tested are directly cultured on the electrode coa...
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IPC IPC(8): G01N33/483C12M1/34
CPCC12M23/12
Inventor 叶学松高天昀王鹏李一乔刘军刘峰
Owner ZHEJIANG UNIV