Supplemented culture medium of proinsulin produced by fermentation and supplemented culture optimization method

Abstract

The invention relates to a supplemented culture medium of proinsulin produced by fermentation and a supplemented culture optimization method, belonging to the field of microorganism engineering. Aiming at the defects of low fermentation density and expression amount, and the like of the prior proinsulin-presenting process by colon bacilli, the invention provides a supplemented culture medium containing a DMEM-high-glucose culture medium and a batch supplementation optimization method for applying the supplemented culture medium to proinsulin fermented by colon bacilli. The culture method has easily controlled process and strong universality and improves the supplemented culture medium of a proinsulin precursor presented by colon bacilli and the supplemented culture optimization method.

Description

technical field

[0001] The invention belongs to the field of microbial engineering, and relates to a feed medium for fermenting and producing proinsulin, and also relates to an optimization method for feed culture of the medium. Background technique

[0002] Diabetes is a chronic endocrine disease. As a specific drug for diabetes, insulin is used in a large amount, with a clinical dosage of several milligrams per day, and the medication takes a long time. Patients with insulin-dependent diabetes generally use insulin for life. But before 1982, all insulin used by humans was derived from animals. Porcine and bovine insulin extracted from animal organs are limited by the source of the organs, and due to the difference in the primary structure, it brings immunogenicity problems and may cause a series of side effects. In 1982, the world's first recombinant drug human insulin (reeombinanthumnainsulin, ht)l came out. Since the primary structure of recombinant human insulin is exa...

Images