Application of ADDA and/or DMTA in preparing medicine for improving expression level of gamma-globin

A technology of expression quantity and globin, which is applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2010-02-24
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There is no report on the use of ADDA or DMTA for the preparation of gamma-globin inducers

Method used

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  • Application of ADDA and/or DMTA in preparing medicine for improving expression level of gamma-globin
  • Application of ADDA and/or DMTA in preparing medicine for improving expression level of gamma-globin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Materials and methods

[0022] 1) compound

[0023] ADDA (adenosine-2', 3'-dialdehyde), DMTA (5'-deoxy-5'(methyl-thio)-adenosine) were purchased from Sigma, USA. ADDA was dissolved in deionized water to make a 10mM stock solution; DMTA was dissolved in DMSO (dimethyl sulfoxide) to make a 0.5M stock solution.

[0024] 2) Cell culture

[0025] Human erythroid K562 cells were cultured at 37°C in RPMI1640 medium, 10% calf serum, 10 5 U / L penicillin, 100mg / L streptomycin, 5% CO 2 . K562 cells were treated with ADDA at a final concentration of 50 μM or 0.1 mM DMTA for 72 hours, and the cells were collected.

[0026] human bone marrow CD34 + Cells were separated with CD34 immunomagnetic beads (Miltenyi Biotec, USA), and 50 μM ADDA or 100 μM DMTA was added, and physiological saline or DMSO (volume ratio 2. Specifically, the cytokines SCF (100 ng / ml), IL-3 (10 ng / ml) and Flt-3 (10 ng / ml) were added to the culture medium for 1 week, and then only the cytokine EPO (5 U / m...

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Abstract

The invention belongs to the technical field of biological medicine, discloses an application of ADDA and / or DMTA in preparing medicine for improving expression level of gamma-globin, and provides a candidate drug for curing anemia caused by beta-thalassemia, sickle-cell anemia, hematopoietic system tumor and hematopoietic dysfunction.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to ADDA (adenosine-2', 3'-diaaldehyde, 2', 3'-dialdehyde adenosine) and / or DMTA (5'-deoxy-5' (methyl-thio) adenosine , 5'-deoxy-5'-methylthiol adenosine), especially the application of ADDA and / or DMTA in the preparation of drugs for improving the expression of γ-globin, and in the preparation of drugs for the treatment of β-thalassemia and sickle cell anemia, hematopoietic system disorders or tumor-induced anemia drugs. Background technique [0002] Hemoglobin in adults consists of two α-globin subunits and two β-globin subunits (α2β2). The human β-like globin gene cluster is located on the short arm of chromosome 11 and contains five sequentially arranged globin genes (ε, G gamma, A γ, δ, β), encoding β-like globin to synthesize hemoglobin. During human individual development, each globin gene is strictly turned on and off according to specific developmental stages and tissue...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7076A61P7/06
Inventor 赵权马驰何颖红
Owner NANJING UNIV
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