Method for preparing rapeseed peptides by mixed fermentation
A technology of mixed bacteria fermentation and rapeseed, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem that the soluble nitrogen content and biological activity need to be further improved, it is difficult to form large-scale industrial production, and the protease activity is affected and other issues, to achieve the effect of environmental protection, low price, and increase the content of soluble nitrogen
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Embodiment 1
[0018] (1) Preparation of liquid fermentation medium
[0019] Nutrient solution composition: glucose 3g, KH 2 PO 4 2g, 1000mL, pH 6.5. Crush the ordinary rapeseed meal to a particle size of about 100 μm, mix the rapeseed meal with nutrient solution at a solid-to-liquid ratio of 1:15 g / mL, and sterilize at 121°C for 30 minutes to obtain a liquid fermentation medium for fermentation inside the tank.
[0020] (2) Liquid fermentation
[0021] ① Preparation of Bacillus subtilis seed solution:
[0022] Incline medium: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, tap water 1000mL, pH7.2-7.4.
[0023] Seed medium: beef extract 3g, peptone 10, NaCl 5g, tap water 1000mL, pH 7.2-7.4.
[0024] Preparation of fermented seeds: Pick two rings of Bacillus subtilis from the activated strain culture slant and inoculate in the seed medium, seal with 8 layers of gauze, culture on a shaking table at 35°C, 120r / min for 24 hours, and the bacteria in the fermentation broth body concentrat...
Embodiment 2
[0049] (1) Preparation of liquid fermentation medium
[0050] Nutrient solution composition: glucose 3g, KH 2 PO 4 2g, 1000mL, pH 6.5. Crush the ordinary rapeseed meal to a particle size of about 100 μm, mix it evenly with the rapeseed meal with a nutrient solution at a solid-to-liquid ratio of 1:25 g / mL, and sterilize at 121°C for 30 minutes to obtain a liquid fermentation medium for fermentation inside the tank.
[0051] (2) Liquid fermentation
[0052] ① Preparation of Bacillus subtilis seed solution:
[0053] Incline medium: beef extract 3g, peptone 10g, NaCl 5, agar 20g, tap water 1000mL, pH7.2-7.4.
[0054] Seed medium: beef extract 3g, peptone 10, NaCl 5g, tap water 1000mL, pH 7.2-7.4.
[0055] Preparation of fermented seeds: Pick two rings of Bacillus subtilis from the activated strain culture slant and inoculate in the seed medium, seal with 8 layers of gauze, culture on a shaking table at 35°C, 120r / min for 24 hours, and the bacteria in the fermentation broth ...
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